In vivo time-course changes in ethanol levels sampled with subcutaneous microdialysis

Alcoholism, Clinical and Experimental Research
Eric A EnglemanWilliam J McBride

Abstract

The objective of this study was to determine time-course changes in in vivo ethanol (EtOH) concentrations using a novel subcutaneous (s.c.) microdialysis sampling technique. The hypothesis to be tested was that EtOH concentrations in the s.c. fluid would reflect blood EtOH concentrations. If this is the case, then s.c. microdialysis could allow a more detailed analysis of changes in in vivo levels of EtOH under different drinking paradigms. Adult male and female Wistar rats and male alcohol-preferring (P) rats were used in this study. A loop-style microdialysis probe was designed for s.c. applications. After initial in vitro characterization, probes were implanted under the skin between the shoulder blades. Animals were allowed to recover 4 to 24 hours prior to microdialysis collection (2.0 microl/min flow rate with isotonic saline). In vivo microdialysis experiments were then conducted to determine (i) the extraction fraction (or clearance) using EtOH no-net-flux (NNF) coupled with the alcohol clamp method, (ii) the dose-response and time-course effects after systemic EtOH administration and to compare with blood EtOH levels, and (iii) the time-course changes in EtOH levels during and after an EtOH drinking episode. In vivo pr...Continue Reading

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Citations

Jun 26, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christina L RubyJ David Glass
Feb 18, 2010·Sensors and Actuators. B, Chemical·C Parks CheneyS O'Connor
Sep 21, 2011·Chronobiology International·Allison J BragerJ David Glass

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