In vivo tissue has non-linear rheological behavior distinct from 3D biomimetic hydrogels, as determined by AMOTIV microscopy

Biomaterials
Benjamin H BlehmKandice Tanner

Abstract

Variation in matrix elasticity has been shown to determine cell fate in both differentiation and development of malignant phenotype. The tissue microenvironment provides complex biochemical and biophysical signals in part due to the architectural heterogeneities found in extracellular matrices (ECMs). Three dimensional cell cultures can partially mimic in vivo tissue architecture, but to truly understand the role of viscoelasticity on cell fate, we must first determine in vivo tissue mechanical properties to improve in vitro models. We employed Active Microrheology by Optical Trapping InVivo (AMOTIV), using in situ calibration to measure in vivo zebrafish tissue mechanics. Previously used trap calibration methods overestimate complex moduli by ∼ 2-20 fold compared to AMOTIV. Applying differential microscale stresses and strains showed that hyaluronic acid (HA) gels display semi-flexible polymer behavior, while laminin-rich ECM hydrogels display flexible polymer behavior. In contrast, zebrafish tissues displayed different moduli at different stresses, with higher power law exponents at lower stresses, indicating that living tissue has greater stress dependence than the 3D hydrogels examined. To our knowledge, this work is the fi...Continue Reading

References

Jun 3, 1994·Cell·K Svoboda, S M Block
Jul 8, 1993·The Journal of Comparative Neurology·A Hansen, E Zeiske
Jul 3, 2002·Nature Reviews. Molecular Cell Biology·Lucy Erin O'BrienKeith E Mostov
Sep 17, 2002·Current Opinion in Cell Biology·Edna CukiermanKenneth M Yamada
Jan 28, 2003·Biophysical Journal·Erwin J G PetermanChristoph F Schmidt
Aug 6, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael J ReddPaul Martin
Oct 27, 2004·Biophysical Journal·M T ValentineD A Weitz
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Apr 12, 2006·Physical Review Letters·J LiuD A Weitz
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Dec 19, 2006·Biophysical Journal·Christopher B RaubSteven C George
Feb 20, 2007·Developmental Biology·Jayoung ChoiJau-Nian Chen
Mar 31, 2007·Nature Methods·Genee Y LeeMina J Bissell
May 24, 2007·Journal of Neurotrauma·Benjamin S ElkinBarclay Morrison
Aug 28, 2007·Cell·Kenneth M Yamada, Edna Cukierman
Dec 11, 2007·Biophysical Journal·Christopher B RaubSteven C George
Oct 14, 2008·Trends in Cell Biology·R Grant Rowe, Stephen J Weiss
Jan 21, 2009·Cancer Metastasis Reviews·Sanjay Kumar, Valerie M Weaver
Nov 26, 2009·Cell·Kandice R LeventalValerie M Weaver
Feb 2, 2010·The Review of Scientific Instruments·Mario FischerKirstine Berg-Sørensen
Feb 18, 2010·Proteomics·Chris S HughesGilles A Lajoie
Jul 2, 2010·Annual Review of Cell and Developmental Biology·Albert J KeungDavid V Schaffer
Sep 30, 2010·Journal of Physics. Condensed Matter : an Institute of Physics Journal·M A KotlarchykA J Putnam
Oct 1, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Rahul C Deo, Calum A MacRae
Feb 24, 2011·International Journal of Cancer. Journal International Du Cancer·Gabriel BentonIrina Arnaoutova
Mar 12, 2011·Advanced Materials·Jason A Burdick, Glenn D Prestwich
Jun 2, 2011·PloS One·Maxwell A KotlarchykElliot L Botvinick
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kandice TannerMina J Bissell
Oct 23, 2012·Nature Nanotechnology·Marija PlodinecCora-Ann Schoenenberger
Dec 1, 2012·Physical Biology·Kandice Tanner
Dec 21, 2012·Tissue Engineering. Part B, Reviews·Matthew B Fisher, Robert L Mauck
Feb 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Benjamin H BlehmPaul R Selvin
Apr 27, 2013·Scientific Reports·UNKNOWN Physical Sciences - Oncology Centers NetworkPei-Hsun Wu
Jul 22, 2014·Nature Methods·Qin TangDavid M Langenau
Apr 17, 2015·Science Translational Medicine·Kandice Tanner, Michael M Gottesman

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Citations

Feb 7, 2017·Journal of Biophotonics·Florian HörnerErez Raz
Oct 11, 2017·Journal of Physics D: Applied Physics·Brian A Camley, Wouter-Jan Rappel
Feb 12, 2019·Scientific Reports·Colin D PaulKandice Tanner
Jul 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jack R StauntonKandice Tanner
Oct 14, 2019·Journal of Biomedical Materials Research. Part a·Benjamin S SpearmanChristine E Schmidt
Jun 25, 2020·Molecular Biology of the Cell·Loïc ChaubetAdam G Hendricks
Apr 4, 2020·Scientific Reports·Valerio VitaliPaolo Minzioni
Dec 17, 2020·Acta Biomaterialia·Helge HerthumIngolf Sack
May 31, 2019·Biomaterials·Sadhana JacksonMichael M Gottesman
Nov 10, 2021·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jakob Ernst Luis JordanIngolf Sack
Jul 8, 2021·Scientific Reports·Randhir KumarCornelia Denz

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