In vivo transcription of a cloned prosimian primate SINE sequence.

Nucleic Acids Research
V K Slagel, Prescott L Deininger

Abstract

SINE transcription was studied by introducing a galago Monomer family member (gal39), into the mouse Ltk- cell line. Cells transiently transfected with gal39 produce gal39-specific RNA polymerase III-directed transcripts. In permanent cell lines gal39 expression was largely shut off. Genomic environment, copy number and tandem repetition of integrated SINE sequences influenced whether or not RNA polymerase III-directed gal39 transcripts were detectable. These transcripts differ in length from the observed endogenous transcript in cultured galago cells which hybridizes to this repetitive DNA family.

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Dec 1, 1992·Current Opinion in Genetics & Development·C Schmid, R Maraia
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·G Vansant, W F Reynolds
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