In vivo transcription of nrdAB operon and of grxA and fpg genes is triggered in Escherichia coli lacking both thioredoxin and glutaredoxin 1 or thioredoxin and glutathione, respectively.

The Journal of Biological Chemistry
R Gallardo-MaduenoC Pueyo

Abstract

We have previously described () that Escherichia coli maintains a balanced supply of deoxyribonucleotides by a regulatory mechanism that up-regulates the levels of ribonucleotide reductase with the lack of its main hydrogen donors thioredoxin, glutaredoxin 1, and glutathione (GSH). By using a semi-quantitative reverse transcription/multiplex polymerase chain reaction fluorescent procedure that enables simultaneous analysis of up to seven mRNA species, we now demonstrate that regulation operates at the transcriptional level. Double mutant cells lacking both thioredoxin and glutaredoxin 1 had increased transcription of the nrdAB operon, as compared with the corresponding wild type parent (maximal induction of 10- and 9-fold for mRNA of nrdA and nrdB genes, respectively). Likewise, a dramatic increase of 36-fold in grxA mRNA was observed in bacteria simultaneously deficient in thioredoxin and GSH (the physiological reductant of all glutaredoxins). The increased expression of the grxA gene in trxA gshA double mutant bacteria was mimicked in trxA single mutant cells by depletion of GSH with diethylmaleate (DEM). This induction of grxA transcription was rapid since maximal increase was detected upon 10 min of DEM exposure. Like grxA ...Continue Reading

References

Nov 24, 1975·Molecular & General Genetics : MGG·P Apontoweil, W Berends
Oct 1, 1992·Molecular Biology of the Cell·L Sun, J A Fuchs
Dec 1, 1992·Journal of Bacteriology·C A BallR C Johnson
Jan 1, 1988·Annual Review of Biochemistry·P Reichard
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Russel, A Holmgren
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Jun 1, 1983·Journal of Bacteriology·P D Hanke, J A Fuchs
Jul 1, 1982·Journal of General Microbiology·N K DavisR N Perham
Sep 1, 1984·Zeitschrift Für Naturforschung. Section C: Biosciences·A M MataJ López-Barea
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·F AslundA Holmgren
Aug 1, 1994·Journal of Bacteriology·L Sun, J A Fuchs
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H Ding, B Demple
Mar 1, 1997·Trends in Biochemical Sciences·P Reichard
Aug 1, 1997·Journal of Bacteriology·F D SetaG Branlant

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Citations

May 10, 2000·The Science of the Total Environment·M CousinouG Dorado
Jul 11, 2002·Free Radical Biology & Medicine·Fernando Cardozo-PelaezJuan R Sánchez-Ramos
Sep 28, 1999·Trends in Plant Science·Y MeyerF Vignols
Jan 30, 2009·The Journal of Biological Chemistry·Farnaz Zahedi Avval, Arne Holmgren
Jun 15, 2006·Antioxidants & Redox Signaling·Stéphanie Gon, Jon Beckwith
Nov 14, 2000·Journal of Bacteriology·M ManchadoC Pueyo
Feb 20, 2003·Journal of Bacteriology·Bjorn VergauwenJozef J Van Beeumen
Mar 1, 2012·PloS One·Agustino Martínez-AntonioMichael A Savageau
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ron OrtenbergJon Beckwith
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·F AslundG Storz
Sep 21, 2010·Free Radical Biology & Medicine·Arne Holmgren, Rajib Sengupta
Sep 7, 2001·Annual Review of Microbiology·D Ritz, J Beckwith
Sep 5, 2003·The Journal of Biological Chemistry·Juan JuradoCarmen Pueyo
Jun 10, 2005·Acta Biochimica Et Biophysica Sinica·Li LuoGuan-Qiao Yu
Mar 15, 2002·The Journal of Biological Chemistry·Aristi PotamitouAlexios Vlamis-Gardikas

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