In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors

Brain Research
Takashi TorashimaHirokazu Hirai

Abstract

Cerebellar Purkinje cells are key elements in motor learning and motor coordination, and therefore, it is important to clarify the mechanisms by which Purkinje cells integrate information and control cerebellar function. Gene transfer into neurons, followed by the assessment of the effects on neural function, is an effective approach for examining gene function. However, this method has not been used fully in the study of the cerebellum because adenovirus vectors, the vectors most commonly used for in vivo gene transfer, have very low affinity for Purkinje cells. In this study, we used a human immunodeficiency virus (HIV)-derived lentiviral vector and examined the transduction profile of the vector in the cerebellum. A lentiviral vector carrying the GFP gene was injected into the cerebellar cortex. Seven days after the injection, Purkinje cells were efficiently transduced without significant influence on the cell viability and synaptic functions. GFP was also expressed, though less efficiently, in other cortical interneurons and Bergmann glias. In contrast to reported findings with other viral vectors, no transduced cells were observed outside of the cerebellar cortex. Thus, when HIV-derived lentiviral vectors were injected int...Continue Reading

References

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Citations

Aug 12, 2010·The Cerebellum·Jean-Pierre LouboutinDavid S Strayer
Dec 18, 2012·The Cerebellum·Clévio NóbregaLuís Pereira de Almeida
Aug 25, 2011·Molecular Pain·Hongwei YuQuinn H Hogan
Aug 25, 2009·Journal of Neuroscience Methods·Alessandro PapaleRiccardo Brambilla
Nov 13, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kazuhiro ShibaHirofumi Mori
Jul 14, 2006·The European Journal of Neuroscience·Takashi TorashimaHirokazu Hirai
Jul 11, 2012·The European Journal of Neuroscience·Jun NishiyamaMichisuke Yuzaki
Sep 8, 2010·Molecular and Cellular Neurosciences·Yohei OhashiYasushi Miyashita
Nov 18, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Chen-Xi ZhengYa-Yun Wang
Aug 24, 2016·Molecular and Cellular Biology·Takeshi ShimizuKazuhiro Ikenaka
Feb 11, 2018·Scientific Reports·Yuki OgawaHirotaka James Okano
Aug 9, 2020·Cerebral Cortex·Aoi TanakaSeiji Hitoshi
Oct 13, 2009·Neurochemical Research·Masahiko Tanaka

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