In vivo transfection of testicular germ cells and transgenesis by using the mitochondrially localized jellyfish fluorescent protein gene

FEBS Letters
Z HuangNorio Nakatsuji


We aimed to introduce foreign DNA into spermatogenic cells in the testis by injection of the DNA encoding jellyfish fluorescent proteins, green fluorescent protein (GFP) and yellow fluorescent protein (YFP) into the seminiferous tubules and in vivo electroporation. We obtained fluorescent spermatozoa only when using the gene of the YFP protein fused to a mitochondrial localization signal peptide. Intracytoplasmic injection into oocytes of these spermatozoa gave fluorescent fetuses and pups. Almost all of the individuals produced from fluorescent spermatozoa were transgenic. We confirmed integration of the gene into chromosomes and its transmission into offspring. This is the first report of gene transfer into germ cells and subsequent production of transgenic offspring.


Jan 5, 2002·Theriogenology·R J Wall
Dec 25, 2002·Theriogenology·A OguraK Mochida
Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Apr 5, 2007·Nature Protocols·Tetsuichiro Saito
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kayoko KuritaNoriyoshi Sakai
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Feb 8, 2003·DNA and Cell Biology·Ann E O Trezise
Aug 24, 2002·Biology of Reproduction·Kentaro YomogidaYoshitake Nishimune
Feb 28, 2003·Biology of Reproduction·Catherine CelebiBernard Jégou
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 17, 2008·Development, Growth & Differentiation·Kentaro Yomgogida
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jul 12, 2002·The Journal of Gene Medicine·Markus DüchlerMathias Müller
Apr 24, 2003·Reproductive Biomedicine Online·Anthony C F Perry
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji
Aug 9, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christopher D PoradaEsmail D Zanjani
Sep 17, 2005·Theriogenology·Sun-A OckGyu-Jin Rho


Mar 1, 1990·Biology of Reproduction·C L ChatotC A Ziomek
Oct 20, 1989·Cell·R L BrinsterR D Palmiter
Apr 1, 1995·Biology of Reproduction·Y Kimura, R Yanagimachi
May 5, 1997·FEBS Letters·M OkabeYoshitake Nishimune
May 15, 1999·Science·A C PerryR Yanagimachi
Jul 15, 1999·Biochemical and Biophysical Research Communications·H S LiuN J Ke
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
May 10, 2000·Nature Medicine·A Martínez-SerranoC Bueno
Jun 16, 2000·FEBS Letters·M NaganoR L Brinster

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