In Vivo Transmission of an IncA/C Plasmid in Escherichia coli Depends on Tetracycline Concentration, and Acquisition of the Plasmid Results in a Variable Cost of Fitness

Applied and Environmental Microbiology
Timothy J JohnsonPeter R Davies

Abstract

IncA/C plasmids are broad-host-range plasmids enabling multidrug resistance that have emerged worldwide among bacterial pathogens of humans and animals. Although antibiotic usage is suspected to be a driving force in the emergence of such strains, few studies have examined the impact of different types of antibiotic administration on the selection of plasmid-containing multidrug resistant isolates. In this study, chlortetracycline treatment at different concentrations in pig feed was examined for its impact on selection and dissemination of an IncA/C plasmid introduced orally via a commensal Escherichia coli host. Continuous low-dose administration of chlortetracycline at 50 g per ton had no observable impact on the proportions of IncA/C plasmid-containing E. coli from pig feces over the course of 35 days. In contrast, high-dose administration of chlortetracycline at 350 g per ton significantly increased IncA/C plasmid-containing E. coli in pig feces (P < 0.001) and increased movement of the IncA/C plasmid to other indigenous E. coli hosts. There was no evidence of conjugal transfer of the IncA/C plasmid to bacterial species other than E. coli. In vitro competition assays demonstrated that bacterial host background substantiall...Continue Reading

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Citations

Nov 23, 2015·Environment International·Johan Bengtsson-Palme, D G Joakim Larsson
Aug 21, 2016·The Science of the Total Environment·Johan Bengtsson-PalmeD G Joakim Larsson
Sep 13, 2019·Journal of Food Protection·Jessica SubiratsEdward Topp
Apr 12, 2016·Journal of Environmental Quality·Murugan SubbiahDouglas R Call
Mar 22, 2018·Canadian Journal of Microbiology·Amanda C Carroll, Alex Wong
Apr 30, 2021·Microbiology and Molecular Biology Reviews : MMBR·Steven L FoleyJing Han
Mar 31, 2021·Antimicrobial Agents and Chemotherapy·Amy T SiceloffNikki W Shariat

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