In vivo two-photon calcium imaging of neuronal networks

Proceedings of the National Academy of Sciences of the United States of America
Christoph StosiekArthur Konnerth

Abstract

Two-photon calcium imaging is a powerful means for monitoring the activity of distinct neurons in brain tissue in vivo. In the mammalian brain, such imaging studies have been restricted largely to calcium recordings from neurons that were individually dye-loaded through microelectrodes. Previous attempts to use membrane-permeant forms of fluorometric calcium indicators to load populations of neurons have yielded satisfactory results only in cell cultures or in slices of immature brain tissue. Here we introduce a versatile approach for loading membrane-permeant fluorescent indicator dyes in large populations of cells. We established a pressure ejection-based local dye delivery protocol that can be used for a large spectrum of membrane-permeant indicator dyes, including calcium green-1 acetoxymethyl (AM) ester, Fura-2 AM, Fluo-4 AM, and Indo-1 AM. We applied this dye-loading protocol successfully in mouse brain tissue at any developmental stage from newborn to adult in vivo and in vitro. In vivo two-photon Ca2+ recordings, obtained by imaging through the intact skull, indicated that whisker deflection-evoked Ca2+ transients occur in a subset of layer 2/3 neurons of the barrel cortex. Thus, our results demonstrate the suitability ...Continue Reading

References

Jan 1, 1990·Annual Review of Cell Biology·Roger Y Tsien, R Y Tsien
Apr 6, 1990·Science·W DenkWatt W Webb
Sep 1, 1989·Pflügers Archiv : European journal of physiology·F A EdwardsT Takahashi
Oct 1, 1988·Physiological Reviews·Amiram GrinvaldRina Hildesheim
Jan 1, 1995·Journal of Neurophysiology·J R Fetcho, D M O'Malley
Jun 22, 1995·Nature·Rafael Yuste, Winfried Denk
Feb 1, 1993·Journal of Neuroscience Methods·Michael J O'DonovanW Yee
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·M E Raichle
Mar 11, 1999·Current Opinion in Neurobiology·A Buonanno, R D Fields
Mar 26, 1999·Cell Calcium·R ChittajalluG L Collingridge
Apr 19, 2000·Nature Neuroscience·Olga GaraschukArthur Konnerth
Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N C SpitzerT M Gomez
Jan 6, 2001·Neuron·T C Südhof
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·Serge CharpakK R Delaney
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Sep 28, 2001·Journal of Neuroscience Methods·M OheimSerge Charpak
Dec 12, 2001·Neuron·B Q MaoRafael Yuste
Jul 24, 2002·Pflügers Archiv : European journal of physiology·Troy W MargrieBert Sakmann
Aug 6, 2002·Neuron·Nikos LogothetisKâmil Ugurbil
Oct 9, 2002·Current Opinion in Neurobiology·Miguel A L Nicolelis, Sidarta Ribeiro

Citations

May 4, 2010·Analytical and Bioanalytical Chemistry·Naoya TakahashiYuji Ikegaya
Jan 15, 2008·European Journal of Nuclear Medicine and Molecular Imaging·Gerhard EichhoffOlga Garaschuk
Mar 22, 2006·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Shy ShohamRonen Segev
Oct 19, 2006·Pflügers Archiv : European journal of physiology·Olga GaraschukArthur Konnerth
Mar 6, 2007·Pflügers Archiv : European journal of physiology·Thomas Nevian, Fritjof Helmchen
Feb 25, 2009·Pflügers Archiv : European journal of physiology·Stefan M WinterSwen Hülsmann
Dec 3, 2011·Pflügers Archiv : European journal of physiology·Dominik Langer, Fritjof Helmchen
Jul 4, 2006·Cell and Tissue Research·Knut HolthoffArthur Konnerth
Oct 14, 2011·Annals of Biomedical Engineering·Tapan P PatelDavid F Meaney
Aug 1, 2009·Journal of Computational Neuroscience·Christoph EchtermeyerV Anne Smith
Jul 26, 2008·Brain Cell Biology·Philipp SchoenenbergerT G Oertner
Mar 23, 2011·Molecular Neurobiology·Daniel W WessonDonald A Wilson
Aug 18, 2010·ACS Chemical Neuroscience·Sarah E CroweGraham C R Ellis-Davies
Jan 23, 2013·Immunology and Cell Biology·Elena ZenaroGabriela Constantin
Mar 11, 2011·Nature·Davi D BockR Clay Reid
Aug 27, 2013·Nature·Sandra J KuhlmanJoshua T Trachtenberg
Apr 12, 2012·Nature Communications·Christine GrienbergerArthur Konnerth
Jan 23, 2009·Nature Methods·Damian J WallaceMazahir T Hasan
Jan 23, 2009·Nature Methods·Marco MankOliver Griesbeck
Oct 23, 2010·Nature Methods·Patrick J DrewDavid Kleinfeld
May 9, 2012·Nature Methods·Kristina SchulzFritjof Helmchen
Dec 30, 2006·Nature Methods·N Marandi, Arthur Konnerth
Aug 30, 2008·Nature Methods·Nathalie L Rochefort, Arthur Konnerth
Jan 30, 2007·Nature Methods·Nicola HeimOliver Griesbeck
Nov 26, 2009·Nature Methods·Christian D Wilms, Michael Häusser
Mar 23, 2005·Nature Methods·Axel NimmerjahnFritjof Helmchen
Nov 22, 2005·Nature Methods·Fritjof Helmchen, Winfried Denk
Dec 5, 2006·Nature Methods·Werner GöbelFritjof Helmchen
Mar 24, 2009·Nature Neuroscience·Emre YaksiRainer W Friedrich
Feb 2, 2010·Nature Neuroscience·Gideon RothschildAdi Mizrahi
Feb 2, 2010·Nature Neuroscience·Sharba BandyopadhyayPatrick O Kanold
Aug 17, 2010·Nature Neuroscience·Spencer L Smith, Michael Häusser
Jan 29, 2011·Nature Neuroscience·Ian H Stevenson, Konrad P Kording
Jul 19, 2011·Nature Neuroscience·Sonja B HoferThomas D Mrsic-Flogel
Jul 12, 2011·Nature Neuroscience·Wolfgang MittmannJason N D Kerr
Jul 12, 2005·Nature Neuroscience·Helmuth AdelsbergerArthur Konnerth
Feb 6, 2013·Nature Nanotechnology·Micha E Spira, Aviad Hai
Apr 5, 2007·Nature Protocols·Shoji KomaiTroy W Margrie
Apr 5, 2007·Nature Protocols·Olga GaraschukArthur Konnerth
Oct 3, 2009·Nature Protocols·Masanori Murayama, Matthew E Larkum
Mar 5, 2011·Nature Protocols·Graham C R Ellis-Davies
Sep 15, 2012·Nature Protocols·Xiaowei ChenArthur Konnerth
Feb 9, 2013·Nature Protocols·Elisa RomanelliMartin Kerschensteiner
May 25, 2013·Nature Protocols·Nathan R WilsonMriganka Sur
Oct 22, 2004·Nature Reviews. Neuroscience·Amiram Grinvald, Rina Hildesheim
May 23, 2006·Nature Reviews. Neuroscience·Thomas Misgeld, Martin Kerschensteiner
Feb 14, 2008·Nature Reviews. Neuroscience·Jason N D Kerr, Winfried Denk
Nov 12, 2009·Nature Reviews. Neuroscience·Brian A Wandell, Stelios Smirnakis
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Bernd KuhnR M Bruno
Oct 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sunil P GandhiMichael P Stryker
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tycho M HooglandSamuel S-H Wang
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chrystel LafontPatrice Mollard
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nathalie L RochefortArthur Konnerth
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bijan Pesaran, David Kleinfeld
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Quan QingCharles M Lieber
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anne ThyssenChristian Lohr
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·William B BarkisMarla B Feller
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Myunghwan ChoiChulhee Choi
Feb 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Edward J BotcherbyTony Wilson
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·John B Issa, Kechen Zhang
May 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marc Aurel BuscheArthur Konnerth
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nicholas H VarvelMathias Jucker
Feb 21, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Megan E CapozziAshwath Jayagopal
Oct 7, 2009·Tissue Engineering. Part C, Methods·Olaf FritzeCsaba Harasztosi
Nov 6, 2012·Brain : a Journal of Neurology·Mohamed M HelmyKai Kaila
Jan 4, 2008·ILAR Journal·Kenneth William Dunn, Timothy Alan Sutton
Jun 7, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jonathan C Horton, Daniel L Adams
Aug 5, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ryota HommaDejan Zecevic
Jan 28, 2009·Photochemistry and Photobiology·Tong YeWarren S Warren
Sep 8, 2011·Journal of Biomedical Optics·Arie KrumholzLihong V Wang
Sep 3, 2011·Science·Xiaoke ChenCharles S Zuker
Jun 27, 2009·Annual Review of Neuroscience·Brian A WiltMark J Schnitzer
Feb 26, 2010·Journal of Neurophysiology·Nikolai C DembrowHarold H Zakon
May 7, 2004·Journal of Neurophysiology·Juergen C JungMark J Schnitzer
Jul 9, 2010·Journal of Neurophysiology·Ilya ValmianskiDavid Kleinfeld
Sep 14, 2012·Journal of Neurophysiology·Abigail S Kalmbach, Jack Waters
Oct 3, 2008·Journal of Neurophysiology·Alex C Kwan
May 2, 2013·Biomedical Engineering Online·Lun-De LiaoNitish V Thakor
Apr 20, 2004·PLoS Biology·Hajime HiraseGyörgy Buzsáki
Jun 23, 2004·PLoS Biology·Mazahir T HasanWinfried Denk
Jul 5, 2007·PLoS Biology·Emre YaksiRainer W Friedrich
Jul 12, 2007·PLoS Biology·Takashi R SatoK Svoboda
Aug 29, 2012·PLoS Computational Biology·Olav StetterTheo Geisel
Aug 9, 2007·PloS One·Dejan Vucinić, Terrence J Sejnowski
Jun 21, 2011·PloS One·Wasim Q MalikEmery N Brown
May 25, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stephen D Van HooserDavid Fitzpatrick
Jul 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cristopher M Niell, Michael P Stryker
Jul 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Samuel W CramerTimothy J Ebner
Jul 21, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lidia ReznichenkoEliezer Masliah
Sep 18, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alex C KwanRonald M Harris-Warrick
Sep 3, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Volodymyr I DzhalaKevin J Staley
Feb 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Esther P YuDouglas A Coulter
Nov 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Isabel BenjumedaEloísa Herrera
Oct 5, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shinghua DingPhilip G Haydon
Sep 4, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Peyman GolshaniCarlos Portera-Cailliau
Aug 20, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Justin Elstrott, Marla B Feller
Sep 11, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zeb L Kurth-NelsonEric A Newman
Sep 9, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Justin A BarnesTimothy J Ebner
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomL L Looger
Dec 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Vincent BoninR Clay Reid
Nov 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel A DombeckDavid W Tank
May 10, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Krystal NizarAnna Devor
Dec 15, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jared M ClemensStephen D Van Hooser
Nov 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David KleinfeldBert Sakmann
Apr 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hajime TakanoDouglas A Coulter
Mar 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hatim A ZariwalaTsai-Wen Chen
Jan 8, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Guisheng ZhongRonald M Harris-Warrick
May 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dierk F ReiffAlexander Borst
Jan 21, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kevin J FordMarla B Feller
Jul 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aaron G BlankenshipMarla B Feller
Sep 2, 2004·Photochemistry and Photobiology·Brett A King, Dennis H Oh
Mar 22, 2012·Journal of Visualized Experiments : JoVE·Andy Y ShihDavid Kleinfeld
Dec 11, 2008·Journal of Visualized Experiments : JoVE·Peyman Golshani, Carlos Portera-Cailliau
Nov 2, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Tizeta TadesseManfred Schmidt
Oct 24, 2012·Neural Plasticity·Giovanni CirilloMichele Papa
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Barbara Lykke LindMartin Lauritzen
Jan 1, 2014·Future Neurology·Shinghua Ding
Jan 11, 2014·Acta Neuropathologica·Bianca BrawekOlga Garaschuk
Sep 26, 2013·Journal of Theoretical Biology·P K Fung, P A Robinson
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiaowei ChenArthur Konnerth
May 20, 2014·Nature Methods·Robert PrevedelAlipasha Vaziri
Sep 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tatjana AtanasijevicAlan Jasanoff
Sep 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Amiram Grinvald
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason N D KerrFritjof Helmchen
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hong XieJi-Song Guan
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Helmuth AdelsbergerArthur Konnerth
Jul 12, 2014·Annual Review of Biomedical Engineering·Melissa R WardenKarl Deisseroth
Jul 29, 2011·Neurocritical Care·C A C WijmanFirst Neurocritical Care Research Conference Investigators
Nov 13, 2007·Journal of Biomedical Optics·Elizabeth M C Hillman
Jan 1, 2008·Journal of Biomedical Optics·Clément RicardBoudewijn van der Sanden
Jan 7, 2009·Journal of Biomedical Optics·Pascale VérantBoudewijn van der Sanden
Jan 5, 2011·Journal of Biomedical Optics·Tingwei QuanShaoqun Zeng
Nov 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Juergen SawinskiJason N D Kerr
Feb 23, 2013·Analytical Chemistry·Todd J HoppeJason B Shear
Feb 26, 2016·Frontiers in Immunology·Enrica PietronigroGabriela Constantin
Feb 29, 2016·Cell Calcium·Ágota ApátiBalázs Sarkadi
Nov 23, 2011·The Journal of Physiology·Christine GrienbergerArthur Konnerth
May 1, 2009·The Journal of Physiology·Florent HaissB Weber
Jul 11, 2012·The Journal of Physiology·Yasumasa OkadaYuji Ikegaya
Dec 23, 2015·Chemical Reviews·Anqi Zhang, Charles M Lieber
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Carsten TischbirekArthur Konnerth
Jul 28, 2010·The Journal of Physiology·Helge C Johannssen, Fritjof Helmchen
Apr 24, 2013·Journal of Neuroscience Methods·Gerardo MauleonDavid T Eddington
May 1, 2013·Journal of Neuroscience Methods·Ji-Geng YanHani S Matloub
Feb 12, 2013·Cell Calcium·Bianca Brawek, Olga Garaschuk
Apr 15, 2006·The Journal of Physiology·Takuya SasakiYuji Ikegaya
Jul 29, 2009·The Journal of Physiology·Veronica Egger, Olga Stroh
Aug 2, 2012·Experimental Neurology·Helge C Johannssen, Fritjof Helmchen
Mar 10, 2012·Biochimica Et Biophysica Acta·Peter Thorn
Mar 1, 2012·Experimental Neurology·Gayane Aramuni, Oliver Griesbeck
Mar 1, 2012·Experimental Neurology·Friederike Siegel, Christian Lohmann
Mar 1, 2012·Experimental Neurology·Olga Garaschuk
Aug 23, 2011·Current Opinion in Neurobiology·Wei-Chung Allen Lee, R Clay Reid
Jul 5, 2011·Journal of Neuroscience Methods·Sabine ScheibeJochen Herms
Jan 7, 2014·Science China. Life Sciences·JingJin DingFeng Shao
Jul 28, 2010·Journal of Neuroscience Methods·Woodrow L ShewDietmar Plenz
Jun 24, 2010·Current Opinion in Neurobiology·Damian J Wallace, Jason N D Kerr
Jun 30, 2009·Journal of Neuroscience Methods·Thomas C HarrisonTimothy H Murphy
Aug 15, 2008·Trends in Molecular Medicine·Nathalie L RochefortArthur Konnerth
Jul 31, 2008·Journal of Structural Biology·Christian PfefferFrançois Légaré
Dec 21, 2007·Current Opinion in Neurobiology·Alan Jasanoff
Jan 22, 2008·Molecular and Cellular Neurosciences·N HarteltS L Mironov
Jul 17, 2012·Neuron·Amos GdalyahuJoshua T Trachtenberg
Jan 12, 2008·Journal of Neuroscience Methods·Karl Wolfgang KafitzChristine R Rose
Aug 28, 2007·Current Opinion in Neurobiology·Kenichi Ohki, R Clay Reid
Jun 26, 2007·NeuroImage·Knut HolthoffOtto W Witte
Apr 25, 2007·Magnetic Resonance Imaging·Gil G Westmeyer, Alan Jasanoff
Apr 10, 2007·Neuroscience Research·Naoya TakahashiYuji Ikegaya
Feb 20, 2007·Cell Calcium·Kenneth Dakin, Wen-Hong Li
Dec 8, 2006·Biochemical and Biophysical Research Communications·Wei ZhouQingming Luo
Oct 3, 2015·Neuroscience Research·Daisuke Miyamoto, Masanori Murayama
Dec 6, 2014·Proteomics·Sascha Sauer, Toni Luge
Oct 13, 2015·Nature Neuroscience·Marc Aurel BuscheArthur Konnerth
Feb 21, 2006·Journal of Neuroscience Methods·Silke D MeierChristine R Rose
Sep 16, 2008·Journal of Neuroscience Methods·David S Greenberg, Jason N D Kerr
Jun 28, 2006·The European Journal of Neuroscience·Ming-Gao ZhaoDiethelm W Richter
Jul 12, 2008·The European Journal of Neuroscience·Rico TaborRainer W Friedrich
Sep 30, 2006·EMBO Reports·Pierre VincentDanièle Paupardin-Tritsch
Nov 18, 2006·Stem Cells·Jelena BanMaria Elisabetta Ruaro
Sep 22, 2010·Journal of Neurochemistry·Tony Kelly, Christine R Rose
May 16, 2012·Journal of Microscopy·M BakalarR S Balaban
Sep 28, 2010·Experimental Physiology·Elena Dreosti, Leon Lagnado
Aug 20, 2010·British Journal of Pharmacology·James T Russell
Feb 8, 2014·Microscopy Research and Technique·Serena BovettiTommaso Fellin
Sep 23, 2014·The Journal of Comparative Neurology·Jeremy F P UllmannCharles Watson
Jan 11, 2014·The European Journal of Neuroscience·Mayumi TadaMasanobu Kano
Mar 8, 2005·Trends in Neurosciences·Alan Jasanoff
Oct 7, 2004·Current Opinion in Neurobiology·Jerome Mertz
Oct 19, 2004·NeuroImage·Jürgen GoldschmidtHenning Scheich
Apr 19, 2005·Biophysical Journal·Haifeng WangJi-Xin Cheng
May 6, 2009·Biophysical Journal·Stephan JunekDetlev Schild
Nov 10, 2015·Nature Neuroscience·Marc Aurel BuscheArthur Konnerth
Jan 31, 2006·Trends in Neurosciences·Thomas KnöpfelWalther Akemann
Sep 1, 2005·Journal of Neuroscience Methods·Jonathan A N FisherArjun G Yodh
Aug 4, 2005·Cell Calcium·Ole H PetersenAlexei Verkhratsky
Apr 12, 2005·Cell Calcium·Rosa CossartRafael Yuste
May 17, 2005·Neuroscience Research·Yuji IkegayaRafael Yuste
Jul 9, 2009·Journal of Neuroscience Methods·Fabienne DupuyClaudio R Lazzari
Jul 8, 2008·Brain Research·Eyal CohenMenahem Segal
May 7, 2014·Neuroscience·M Joshua, S G Lisberger
Nov 19, 2013·Neuron·Hiroyuki K KatoTakaki Komiyama
Apr 12, 2016·The Journal of Clinical Investigation·Sun Kwang KimJunichi Nabekura
Apr 8, 2015·The European Journal of Neuroscience·Stephan DirenbergerLars Kunz
Mar 17, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marc Aurel Busche, Arthur Konnerth
Aug 25, 2015·Frontiers in Neural Circuits·Dylan R MuirBjörn M Kampa
Jan 16, 2014·International Journal of Psychophysiology : Official Journal of the International Organization of Psychophysiology·Mark A Bee
Feb 2, 2013·Journal of Neuroscience Methods·Abduqodir H ToychievBotir T Sagdullaev
Jun 9, 2012·Neurobiology of Disease·Kyle P LillisJohn A White
Apr 1, 2015·ELife·Mayeul CollotJean-Maurice Mallet
Oct 27, 2009·Current Opinion in Neurobiology·Benjamin F Grewe, Fritjof Helmchen
Nov 9, 2010·Biochimica Et Biophysica Acta·Gerhard EichhoffOlga Garaschuk
Oct 19, 2010·Journal of Neuroscience Methods·Suzie DufourFlorin Amzica
Jul 19, 2011·NeuroImage·Christophe M Lamy, Jean-Yves Chatton
Jul 11, 2015·Nature Reviews. Neuroscience·Rafael Yuste
Apr 17, 2015·Current Opinion in Neurobiology·Simon PeronK Svoboda
Oct 30, 2007·Neuron·Leonard E White, David Fitzpatrick
Oct 30, 2007·Neuron·Carl C H Petersen
May 5, 2009·Neuron·Aaron G BlankenshipMarla B Feller
Apr 16, 2013·Neuron·Carl C H Petersen, Sylvain Crochet
Mar 18, 2008·Neuron·Liqun LuoK Svoboda
Sep 26, 2009·Neuron·Eran A MukamelMark J Schnitzer
Apr 20, 2007·Neuron·Padraig GleesonR Angus Silver
May 19, 2009·Neuron·Axel NimmerjahnMark J Schnitzer
Aug 11, 2010·Neuron·Mark Hübener, Tobias Bonhoeffer
Aug 13, 2011·Neuron·Nathalie L RochefortArthur Konnerth
Sep 16, 2003·Neuron·Troy W MargrieMichael Brecht
Mar 31, 2005·Current Biology : CB·Mark Hübener, Tobias Bonhoeffer
Jan 13, 2012·Current Opinion in Neurobiology·Jason N D Kerr, Axel Nimmerjahn
Dec 13, 2006·NeuroImage·Javier Díez-GarcíaThomas Knöpfel
Sep 10, 2011·Neuron·Rafael Yuste
Sep 10, 2011·Neuron·Gabor C Petzold, Venkatesh N Murthy
Mar 13, 2012·Neuron·Christine Grienberger, Arthur Konnerth
Jun 5, 2007·NeuroImage·K B BlagoevE Halgren
Jan 24, 2012·Current Biology : CB·Friederike SiegelChristian Lohmann
Apr 10, 2015·Neuron·Elizabeth J O HamelMark J Schnitzer
Apr 23, 2016·Journal of Neuroscience Methods·Gergely SilasiTimothy H Murphy
Dec 3, 2014·Journal of Neuroscience Methods·Mohammad I K HamadPetra Wahle
Mar 19, 2014·Cell·Yu FuMichael P Stryker
Oct 26, 2010·Journal of Neuroscience Methods·Wolfgang SteinPeter Andras
Jun 10, 2008·Journal of Neuroscience Methods·Kyle P LillisJerome Mertz
Dec 17, 2014·Journal of Neuroscience Methods·Serena Bovetti, Tommaso Fellin
Jan 3, 2006·Biophysical Journal·Tsai-Wen ChenDetlev Schild
Jul 22, 2009·Biophysical Journal·Joshua T VogelsteinLiam Paninski
Jul 9, 2014·Trends in Neurosciences·Patrick O KanoldDaniel B Polley
May 18, 2010·Biochimica Et Biophysica Acta·Laura ContrerasTullio Pozzan
Apr 24, 2007·Biophysical Journal·François LégaréBjorn R Olsen
Dec 30, 2014·Frontiers in Molecular Neuroscience·Gerard J BroussardLin Tian
Dec 17, 2014·Frontiers in Neural Circuits·Christina K KimRebecca D Burdine
Jun 12, 2014·Frontiers in Computational Neuroscience·Jorrit S MontijnCyriel Ma Pennartz
Aug 26, 2014·Frontiers in Neural Circuits·Mark MazurekStephen D Van Hooser
Nov 27, 2014·Frontiers in Cellular Neuroscience·Christopher J Roome, Bernd Kuhn
Dec 3, 2014·Biological cybernetics·Jon Oñativia, Pier Luigi Dragotti
Nov 30, 2014·Trends in Neurosciences·Christine GrienbergerArthur Konnerth
May 7, 2016·Neuron·Lucas TheisMatthias Bethge
Oct 11, 2016·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Jing MenChao Zhou
Jun 9, 2016·Nature Chemical Biology·Hiroki MaedaKazuya Kikuchi
Mar 5, 2016·Allergology International : Official Journal of the Japanese Society of Allergology·Naoto Kawakami
Jun 29, 2016·Pflügers Archiv : European journal of physiology·Ina Maria Schießl, Hayo Castrop
Jul 6, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marc Aurel Busche, Arthur Konnerth
Jul 14, 2016·Cerebral Cortex·Kuan ZhangXiaowei Chen
Jun 28, 2016·Nature Biotechnology·Jeffrey N StirmanSpencer L Smith
Jul 28, 2016·Nature Neuroscience·Chen RanXiaoke Chen
Aug 17, 2016·The European Journal of Neuroscience·Masatoshi Kasai, Tadashi Isa
Sep 14, 2016·Cerebral Cortex·Alexander van der BourgFritjof Helmchen
Oct 6, 2016·Current Biology : CB·Niels NiethardTakashi R Sato
Oct 6, 2016·Neuroscience·Feng WangYves De Koninck
Jan 6, 2017·Scientific Reports·Serena BovettiTommaso Fellin
Feb 20, 2016·PLoS Computational Biology·Vahid RahmatiStefan J Kiebel
Jun 19, 2010·Neurosurgery·Yong WangSusumu Terakawa
Jun 28, 2016·PLoS Computational Biology·Jan AntolikThomas D Mrsic-Flogel
Mar 21, 2017·Nature Methods·Alexander SongDavid W Tank
Apr 7, 2017·Neuron·Jordan P HammRafael Yuste
Apr 6, 2017·Cerebral Cortex·Jingcheng LiXiaowei Chen
Oct 30, 2015·Scientific Reports·Joon-Hyuk LeeSooyoung Chung
May 1, 2016·Trends in Neurosciences·Douglas StoraceBradley J Baker
Oct 23, 2014·Scientific Reports·Hiroaki TakeharaTakanori Ichiki
Oct 22, 2008·Methods : a Companion to Methods in Enzymology·R Madelaine ParedesJames D Lechleiter
Jun 11, 2008·Optics Express·Christoph J EngelbrechtFritjof Helmchen
Nov 22, 2016·Nature Communications·Luc EstebanezJean-François Léger
Apr 1, 2017·Nature Methods·Weijian Yang, Rafael Yuste
Jan 15, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mauro C X PintoRodrigo R Resende
Feb 2, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Andy Y ShihDavid Kleinfeld
Jul 23, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Ian R Winship, Timothy H Murphy
Oct 14, 2017·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Franziska AlbersCornelius Faber
Jul 29, 2003·Pflügers Archiv : European journal of physiology·E BrusteinArthur Konnerth
Dec 7, 2017·Frontiers in Computational Neuroscience·Yaoyu ZhangDavid Cai
May 12, 2018·Nature Protocols·Simon WeilerVolker Scheuss
Nov 14, 2017·Journal of Neural Engineering·Renaud SchuckSimon R Schultz
Sep 5, 2017·Reports on Progress in Physics·Helen MillerMark C Leake
Jan 1, 2017·Light, Science & Applications·Evan P PerilloAndrew K Dunn
Dec 14, 2011·Nature·Johannes J LetzkusAndreas Lüthi
Apr 5, 2013·Nature·Ho KoThomas D Mrsic-Flogel
Jun 9, 2012·Nature·Thomas D Mrsic-Flogel, Tobias Bonhoeffer
May 9, 2014·Nature·Andrew J PetersTakaki Komiyama
Jun 11, 2016·Experimental & Molecular Medicine·Jeong Ho Lee
Dec 15, 2017·PLoS Computational Biology·Dylan R MuirBjörn M Kampa
Mar 11, 2011·Nature·Kevin L BriggmanWinfried Denk
Jun 27, 2018·Current Protocols in Cytometry·Claire E J Cheetham
May 25, 2012·European Journal of Immunology·Bernd SchwendeleOlga Garaschuk
Apr 3, 2014·Cold Spring Harbor Protocols·Kenichi Ohki, R Clay Reid
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Oct 21, 2004·European Journal of Immunology·Eberhard TutschMarkus Hoth
Oct 29, 2004·Journal of Neurophysiology·Agnes BonnotMichael J O'Donovan
Nov 1, 2018·Current Protocols in Cytometry·Piyusha S GadeChih-Yuan Chuang
Apr 3, 2008·Developmental Neurobiology·Michael J O'DonovanPeter Wenner
Apr 15, 2009·Optics Express·Christoph J EngelbrechtFritjof Helmchen
Oct 16, 2009·Nature·Massimo Scanziani, Michael Häusser
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Chommanad LerdkraiOlga Garaschuk
Jun 22, 2006·The Journal of Biological Chemistry·Victoria F SafiulinaRashid Giniatullin
Sep 8, 2006·American Journal of Physiology. Cell Physiology·Frank FunkeMichael Müller
Aug 30, 2016·Nature Neuroscience·Na JiSpencer L Smith
Aug 30, 2016·Nature Neuroscience·Kenneth D HarrisSpencer L Smith
Feb 9, 2007·Journal of Neurophysiology·Aren BorgdorffCarl C H Petersen
Feb 17, 2007·Journal of Neurophysiology·J M WilsonR M Brownstone
Dec 13, 2007·Physiology·Werner Göbel, Fritjof Helmchen
Feb 20, 2015·Nature Communications·Yury KovalchukOlga Garaschuk
Jul 4, 2008·Journal of Neurophysiology·Takuya SasakiYuji Ikegaya
Apr 28, 2009·Physical Review Letters·O MarreA Destexhe
Aug 4, 2009·The Journal of Physiology·Patrick J Whelan
Apr 30, 2010·Nature·Hongbo JiaArthur Konnerth
Jun 28, 2011·Nature·Xiaowei ChenArthur Konnerth
Oct 20, 2010·Somatosensory & Motor Research·Cornelius SchwarzChristian Waiblinger
Jul 8, 2011·Journal of Neurophysiology·Romain FranconvilleIsabel Llano
Jun 23, 2019·Journal of Neuroscience Research·Chiara CiriachiMattias Rickhag
Jul 19, 2013·Journal of Neural Engineering·Jon OñativiaPier Luigi Dragotti
Dec 29, 2013·Cold Spring Harbor Protocols·Michael Häusser, Troy W Margrie
Apr 18, 2014·Journal of Neurophysiology·Justin ElstrottDaniel E Feldman
May 20, 2014·Organic & Biomolecular Chemistry·Dokyoung KimKyo Han Ahn
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Sep 28, 2007·Journal of Neurophysiology·Werner Göbel, Fritjof Helmchen
Mar 10, 2017·Physiological Reviews·Mikhail A Lebedev, Miguel A L Nicolelis
Apr 26, 2017·Frontiers in Neural Circuits·Quanchao ZhangXiaowei Chen
Feb 1, 2018·Science Signaling·Nathan A SmithMaiken Nedergaard
Oct 13, 2010·Current Protocols in Cytometry·Krishnan PadmanabhanJames A J Fitzpatrick
Apr 29, 2015·Journal of Biophotonics·Moritz KneippDaniel Razansky
Nov 11, 2017·Brain Structure & Function·Jiangheng GuanXiang Liao
Aug 11, 2020·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·James A SimmonsAndrea Megela Simmons
Aug 23, 2020·Brain Sciences·Lauren C Smith, Adam Kimbrough
Jul 20, 2012·Physiological Reviews·Luc Leybaert, Michael J Sanderson
Jan 2, 2015·Neurophotonics·Benjamin A SuterGordon M G Shepherd
Mar 18, 2015·Biomedical Optics Express·Xiuli LiuShaoqun Zeng
Sep 12, 2017·Journal of Integrative Neuroscience·Tielin ZhangBo Xu
Jan 10, 2018·Scientific Reports·Meng WangXiang Liao
Mar 8, 2018·Current Protocols in Immunology·Monica Manglani, Dorian B McGavern
Aug 15, 2018·Journal of Integrative Neuroscience·Satoshi Iwasaki, Yuji Ikegaya
Aug 4, 2018·Frontiers in Aging Neuroscience·Patricia KellyBrian J Bacskai
Oct 27, 2018·Nature Neuroscience·Jiwei YaoXiaowei Chen
Oct 23, 2019·Frontiers in Cellular Neuroscience·Antonia Beiersdorfer, Christian Lohr
Apr 22, 2020·Nature Communications·Vishnudev RamachandraJason N D Kerr
Jul 2, 2015·Advanced Materials·Adam WilliamsonGeorge G Malliaras
Oct 28, 2015·Biomedical Optics Express·Qingchun GuoLing Fu
May 26, 2016·Journal of Neuroinflammation·Jerel A FieldsEliezer Masliah
Oct 28, 2015·Biomedical Optics Express·Vini GautamVincent R Daria
Apr 7, 2017·Frontiers in Neural Circuits·Mónica López-HidalgoJames Schummers
Dec 21, 2016·The Journal of Physiology·Arthur KonnerthArthur Konnerth
Dec 7, 2016·Science China. Life Sciences·Tianwen Huang, Qinyong Ye
Jun 8, 2017·Scientific Reports·Ran DingXiaowei Chen
Oct 10, 2018·Scientific Reports·Oliver BraubachYunsook Choi
Jan 20, 2019·Nature Chemical Biology·Wenjing WangAlice Y Ting
Sep 2, 2020·Nature Communications·Meng WangXiaowei Chen
Aug 5, 2005·Journal of Neurophysiology·Megan R SullivanSamuel S-H Wang
Feb 25, 2011·Journal of Neurophysiology·Kevin L Briggman, Thomas Euler
Jul 30, 2010·Journal of Neurophysiology·Claudia Roth-AlpermannMichael Brecht
Jul 14, 2011·Biomedical Optics Express·Benjamin F GreweFritjof Helmchen
May 11, 2017·PloS One·Yahya KarimipanahRalf Wessel
Jul 16, 2019·Small·Huiting LuMenglian Wei
Feb 23, 2019·Frontiers in Behavioral Neuroscience·Cory T MillerPartha Mitra
Jul 21, 2019·Nature Communications·Hsing-Jung Chen-EngererArthur Konnerth
Nov 16, 2019·Lasers in Medical Science·Pranoy Sahu, Nirmal Mazumder
Oct 3, 2020·Scientific Reports·Chao J LiuPrakash Kara
Jul 7, 2020·Frontiers in Neuroscience·Zeynep I GunesSabine Liebscher
Oct 6, 2009·Current Protocols in Neuroscience·Douglas R FieldsMichael J O'Donovan
Nov 13, 2015·Journal of Neurophysiology·Michael A GaffieldJason M Christie
Oct 30, 2014·Molecular Neurobiology·Giovanni CirilloMichele Papa
Oct 29, 2015·Journal of Biomedical Optics·Ye ChenJonathan T C Liu
Jan 31, 2017·Frontiers in Biology·Rachel Babij, Natalia De Marco Garcia
Aug 10, 2019·Science·Benedikt ZottArthur Konnerth
May 28, 2016·Biomedical Optics Express·Peter RupprechtRainer W Friedrich
Apr 4, 2009·Journal of Biophotonics·Katja Schenke-Layland
Jul 19, 2017·Biomedical Optics Express·Fabian F VoigtUrsula Keller
Mar 28, 2017·F1000Research·James N SleighGiampietro Schiavo
Apr 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qiang YeQinghua Lu
Oct 22, 2020·Scientific Reports·Huu HoangKeisuke Toyama
Nov 17, 2020·Frontiers in Cellular Neuroscience·Ina GasterstädtPetra Wahle
Dec 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Saikat RayRobert Konrad Naumann
Dec 19, 2020·Current Opinion in Neurobiology·Stephen L KeeleyJonathan W Pillow
Oct 27, 2020·Computational and Structural Biotechnology Journal·Adrià Tauste Campo
May 30, 2020·Neuroscience Research·Tatsuo K Sato
Oct 24, 2020·Current Biology : CB·Ramanujan SrinathKristina J Nielsen
Jan 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yayao MaLiang Gao
Dec 15, 2020·Biomaterials·Yihang ChenGuosong Hong
Dec 2, 2020·Journal of Neuroscience Methods·Brenton T LaingYeka Aponte
Dec 15, 2019·Brain, Behavior, and Immunity·Karin RiesterOlga Garaschuk
Dec 19, 2020·Cell Death and Differentiation·Lohans PedreraAna J García-Sáez
Jan 24, 2021·Journal of Neuroscience Methods·Zhenkun ZhangKai Wang
Dec 16, 2020·The Journal of Chemical Physics·Jurick LahiriMarcos Dantus
Mar 5, 2021·Biomedical Optics Express·A FernándezA J Verhoef
Feb 20, 2021·Neuroscience Research·Masanari OndaFumitaka Osakada
Aug 17, 2020·Journal of Neuroscience Methods·Brice De La CrompeIlka Diester
Feb 22, 2017·Neuron·Ming LiShiming Tang
Apr 17, 2018·Current Opinion in Neurobiology·Rafael Yuste, Rafael Yuste
Mar 14, 2021·Nature Reviews. Neuroscience·Daniel ChoquetJean-Baptiste Sibarita
Jun 29, 2017·Cell Reports·Michael WenzelRafael Yuste
Jan 18, 2018·Current Opinion in Biomedical Engineering·David R MillerAndrew K Dunn
Mar 27, 2021·Frontiers in Endocrinology·Shiuhwei ChenWen-Hong Li
Oct 6, 2018·Trends in Neurosciences·Shane M McMahon, Meyer B Jackson

Related Concepts

Brain
Electric Stimulation Technique
Fluorochromes
Mice, Inbred BALB C
Immunofluorescence Microscopy
Neural Networks (Anatomic)
Physical Stimulation
Staining and Labeling
Photons
Glutamic Acid, (D)-Isomer

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jason N D KerrFritjof Helmchen
Proceedings of the National Academy of Sciences of the United States of America
Amiram Grinvald
© 2021 Meta ULC. All rights reserved