In vivo vascularization of MSC-loaded porous hydroxyapatite constructs coated with VEGF-functionalized collagen/heparin multilayers

Scientific Reports
Kai JinChangyou Gao

Abstract

Rapid and adequate vascularization is vital to the long-term success of porous orbital enucleation implants. In this study, porous hydroxyapatite (HA) scaffolds coated with vascular endothelial growth factor (VEGF)-functionalized collagen (COL)/heparin (HEP) multilayers (porosity 75%, pore size 316.8 ± 77.1 μm, VEGF dose 3.39 ng/mm(3)) were fabricated to enhance vascularization by inducing the differentiation of mesenchymal stem cells (MSCs) to endothelial cells. The in vitro immunofluorescence staining, quantitative real-time polymerase chain reaction (qRT-PCR), and western blotting results demonstrated that the expression of the endothelial differentiation markers CD31, Flk-1, and von Willebrand factor (vWF) was significantly increased in the HA/(COL/HEP)5/VEGF/MSCs group compared with the HA/VEGF/MSCs group. Moreover, the HA/(COL/HEP)5 scaffolds showed a better entrapment of the MSCs and accelerated cell proliferation. The in vivo assays showed that the number of newly formed vessels within the constructs after 28 d was significantly higher in the HA/(COL/HEP)5/VEGF/MSCs group (51.9 ± 6.3/mm(2)) than in the HA (26.7 ± 2.3/mm(2)) and HA/VEGF/MSCs (38.2 ± 2.4/mm(2)) groups. The qRT-PCR and west rn blotting results demonstrated...Continue Reading

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Citations

Oct 30, 2016·ACS Applied Materials & Interfaces·Ana M FerreiraMark A Birch
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Francesco Baino, Isabel Potestio
Feb 12, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Martha SchamelAnja Lode
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Aug 13, 2018·ACS Biomaterials Science & Engineering·Negar Hassani BesheliMehdi Farokhi
Jun 11, 2018·ACS Biomaterials Science & Engineering·Tao ShenChangyou Gao

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Methods Mentioned

BETA
quartz crystal microbalance
flow cytometry
ELISA
scanning electron microscopy
fluorescence-activated cell sorter
PCR
electrophoresis

Software Mentioned

SEM
Origin
ImageJ

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