In vivo visualization of locally transplanted mesenchymal stem cells in the severely injured muscle in rats

Tissue Engineering. Part a
Tobias WinklerGeorg Matziolis

Abstract

Autologous mesenchymal stem cells (MSCs) have been shown to improve the functional outcome after skeletal muscle trauma. The mechanisms behind this improvement have to be answered prior to a future clinical application. We investigated for the first time the in vivo distribution and behavior of MSCs after local transplantation into a severely injured muscle with magnetic resonance imaging (MRI). Autologous rat MSCs were labeled with very small iron oxide nanoparticles (VSOPs) and transplanted into the soleus muscle 1 week after an open crush injury. Distribution and migration of the cells were evaluated in vivo over time by the repeated performance of high-resolution MRI at 7 T. Three and 6 weeks after transplantation, the muscles were histologically analyzed. The labeled MSCs could be visualized inside the traumatized muscles 24 h after transplantation showing characteristic signal reductions in T2*-weighted sequences. The hypointense signal could be followed over 6 weeks and could be easily discriminated from the structures of the injured muscle. The cell pools did not migrate inside the muscle and showed a decrease in volume over time. Prussian blue-stained histologic sections showed a topographical correlation of the respec...Continue Reading

References

May 10, 1996·The Journal of Biological Chemistry·C E Stewart, P Rotwein
Oct 1, 1996·Journal of Cellular Physiology·C E StewartP Rotwein
Feb 8, 2000·Clinical Orthopaedics and Related Research·C KasemkijwattanaJ Huard
Mar 4, 2000·The Journal of Bone and Joint Surgery. British Volume·J MenetreyJ Huard
Jul 18, 2000·Experimental Cell Research·E El FahimeJ P Tremblay
Jul 31, 2001·The American Journal of Sports Medicine·K FukushimaJ Huard
Sep 5, 2001·Nature Cell Biology·J G TomaF D Miller
Sep 11, 2002·Neuromuscular Disorders : NMD·Terry Partridge
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jul 2, 2003·Cells, Tissues, Organs·Daniel A De UgarteMarc H Hedrick
Oct 11, 2003·Experimental Hematology·Katarina Le BlancOlle Ringdén
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Frank P Barry, J Mary Murphy
Jul 13, 2004·Nuclear Medicine and Biology·Weimin DingJiahe Tian
Dec 14, 2004·Transplantation·Kevin S CahillGlenn A Walter
Mar 1, 2005·Developmental Biology·Adam T PalermoHelen M Blau
Mar 15, 2005·Biology of the Cell·Young-Sup YoonHeather Scadova
Nov 1, 2005·Nature Biotechnology·I Jolanda M de VriesCarl G Figdor
Apr 29, 2006·Circulation Research·Keiichi Fukuda, Shinsuke Yuasa
Jul 11, 2006·Life Sciences·T TallhedenE Omerovic
Aug 16, 2006·Neuro-degenerative Diseases·Eva Syková, Pavla Jendelová
Sep 5, 2006·Current Problems in Diagnostic Radiology·Khaled M ElsayesDavid A Rubin
Mar 21, 2007·Journal of Cellular and Molecular Medicine·Denitsa DochevaMatthias Schieker
Mar 28, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bruno PéaultJohnny Huard
Jul 7, 2007·Leukemia & Lymphoma·Danièle NoëlChristian Jorgensen

❮ Previous
Next ❯

Citations

Feb 25, 2014·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Seigo OshimaMitsuo Ochi
Oct 12, 2010·Biomaterials·Eliza L S FongStuart B Goodman
Jul 5, 2012·Journal of Tissue Engineering and Regenerative Medicine·Tobias WinklerCarsten Perka
May 19, 2018·Journal of Tissue Engineering and Regenerative Medicine·Marissa A RuehleJarrod A Call
Sep 4, 2013·Regenerative Medicine·Benjamin M DaviesAndrew Carr
Aug 26, 2016·Immunopharmacology and Immunotoxicology·Mona A M HelalFatma A Abu Zahra
Aug 29, 2012·The Open Orthopaedics Journal·Philipp von RothTobias Winkler
Dec 18, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Suyan BianHepeng Yu
Mar 14, 2018·International Journal of Molecular Sciences·F Andrea SassKatharina Schmidt-Bleek
Sep 20, 2018·Journal of Cachexia, Sarcopenia and Muscle·Tobias WinklerHans-Dieter Volk
Dec 22, 2016·Tissue Engineering. Part B, Reviews·Shama R IyerRichard M Lovering

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.