In vivo X-ray digital subtraction and CT angiography of the murine cerebrovasculature using an intra-arterial route of contrast injection.

AJNR. American Journal of Neuroradiology
Giovanna FigueiredoMarc A Brockmann

Abstract

Investigation of the anatomy, patency, and blood flow of arterial and venous vessels in small animal models of cerebral ischemia, venous thrombosis, or vasospasm is of major interest. However, due to their small caliber, in vivo examination of these vessels is technically challenging. Using micro-CT, we compared the feasibility of in vivo DSA and CTA of the murine cerebrovasculature using an intra-arterial route of contrast administration. The ECA was catheterized in 5 C57BL/6J mice. During intra-arterial injection of an iodized contrast agent (30 μL/1 sec), DSA of the intra- and extracranial vessels was performed in mice breathing room air and repeated in hypoxic/hypercapnic mice. Micro-CTA was performed within 20 seconds of intra-arterial contrast injection (220 μL/20 sec). Image quality of both methods was compared. Radiation dose measurements were performed with thermoluminescence dosimeters. Both methods provided high-resolution images of the murine cerebrovasculature, with the smallest identifiable vessel calibers of ≤ 50 μm. Due to its high temporal resolution of 30 fps, DSA allowed identification of anastomoses between the ICA and ECA by detection of retrograde flow within the superficial temporal artery. Micro-CTA duri...Continue Reading

References

Jan 1, 1972·Journal of Neuropathology and Experimental Neurology·W I Rosenblum
Jul 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·F C BaroneR N Willette
Nov 5, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Z ZhangA Goussev
Jul 16, 2002·Neurological Research·Augusto ParraDavid S Warner
Jan 29, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Dong-Eog KimChing-Hsuan Tung
Jun 3, 2006·Stroke; a Journal of Cerebral Circulation·Keiji KidoguchiKeiji Umetani
Dec 13, 2006·Medical Physics·Ming De LinG Allan Johnson
Jun 9, 2007·Medical Physics·Cristian T BadeaG Allan Johnson
Nov 23, 2007·Biochemical Society Transactions·J Anckar, L Sistonen
Mar 13, 2008·IEEE Transactions on Bio-medical Engineering·Ming de LinG Allan Johnson
Mar 28, 2008·Physics in Medicine and Biology·Youngho SeoBruce H Hasegawa
Sep 2, 2008·Physics in Medicine and Biology·C T BadeaG A Johnson
Feb 14, 2009·Stroke; a Journal of Cerebral Circulation·Sebastian J SchambachMarc A Brockmann
Jun 19, 2009·NMR in Biomedicine·Christof BaltesMarkus Rudin
Aug 19, 2009·Brain Research·Mohammed SabriR Loch Macdonald
Sep 24, 2009·Methods : a Companion to Methods in Enzymology·Sebastian J SchambachMarc A Brockmann
Nov 11, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Gabriel P HowlesG Allan Johnson
May 22, 2010·AJNR. American Journal of Neuroradiology·A BuhalogC M Strother
Jun 3, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Mohammed SabriRobert Loch Macdonald

❮ Previous
Next ❯

Citations

Jul 25, 2013·Journal of Neuroscience Methods·Martin JuenemannTibo Gerriets
Dec 6, 2012·Chemical Reviews·Hrvoje Lusic, Mark W Grinstaff
Jul 13, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kristof GovaertsUwe Himmelreich
Mar 19, 2021·The Journal of International Medical Research·Li ChenShan Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.