In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers

Scientific Reports
Eva VerguchtLaszlo Vincze

Abstract

We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability.

References

Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·K Svoboda, S M Block
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·R Neutze, J Hajdu
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y NishimuraT Kuroiwa
Nov 5, 1999·Biophysical Journal·K C NeumanS M Block
Jul 13, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Stefan P Hau-RiegeAbraham Szoke
Nov 13, 2004·Analytical Chemistry·Laszlo VinczeFreddy Adams
Sep 15, 2006·American Journal of Physics·Matthew J Lang, Steven M Block
Jan 17, 2008·Chemical Society Reviews·Gérard Férey
Mar 1, 2008·Annual Review of Biochemistry·Jeffrey R MoffittCarlos Bustamante
Apr 10, 2010·Water Research·Meng Nan ChongChris Saint
Mar 1, 2009·Journal of Electron Spectroscopy and Related Phenomena·M R HowellsD Starodub
Feb 22, 2011·Journal of Synchrotron Radiation·Gavin B M VaughanAnatoly Snigirev
Feb 7, 2012·Physical Review Letters·Filip BeunisDmitri Petrov
May 18, 2013·Analytical and Bioanalytical Chemistry·B De SamberL Vincze
Dec 30, 2014·Analytical and Bioanalytical Chemistry·Eva VerguchtFrank Vanhaecke

❮ Previous
Next ❯

Citations

Jul 7, 2018·Journal of Synchrotron Radiation·Jan David NicolasTim Salditt
Mar 31, 2018·Optics Letters·Min JiangXuping Zhang
Feb 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yuan GaoLinda Young
Aug 9, 2017·ACS Nano·Clément Y J Hémonnot, Sarah Köster

❮ Previous
Next ❯

Methods Mentioned

BETA
X-Ray
optical tweezers
chip

Software Mentioned

IDL

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.