In1-ghrelin splicing variant is overexpressed in pituitary adenomas and increases their aggressive features

Scientific Reports
Alejandro Ibáñez-CostaRaul M Luque

Abstract

Pituitary adenomas comprise a heterogeneous subset of pathologies causing serious comorbidities, which would benefit from identification of novel, common molecular/cellular biomarkers and therapeutic targets. The ghrelin system has been linked to development of certain endocrine-related cancers. Systematic analysis of the presence and functional implications of some components of the ghrelin system, including native ghrelin, receptors and the recently discovered splicing variant In1-ghrelin, in human normal pituitaries (n = 11) and pituitary adenomas (n = 169) revealed that expression pattern of ghrelin system suffers a clear alteration in pituitary adenomasas compared with normal pituitary, where In1-ghrelin is markedly overexpressed. Interestingly, in cultured pituitary adenoma cells In1-ghrelin treatment (acylated peptides at 100 nM; 24-72 h) increased GH and ACTH secretion, Ca(2+) and ERK1/2 signaling and cell viability, whereas In1-ghrelin silencing (using a specific siRNA; 100 nM) reduced cell viability. These results indicate that an alteration of the ghrelin system, specially its In1-ghrelin variant, could contribute to pathogenesis of different pituitary adenomas types, and suggest that this variant and its related ghr...Continue Reading

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Citations

Apr 20, 2016·Endocrinología y nutrición : órgano de la Sociedad Española de Endocrinología y Nutrición·Raúl M LuqueUNKNOWN investigadores del REMAH
Sep 3, 2016·The Journal of Endocrinology·Alejandro Ibáñez-CostaJusto P Castaño
Nov 26, 2015·Scientific Reports·David SchultRolf Buslei
Nov 10, 2015·Scientific Reports·Juan F Martín-RodríguezDavid A Cano
Aug 31, 2017·Molecular Cancer·Daniel Hormaechea-AgullaRaúl M Luque
Sep 29, 2018·The Journal of Clinical Endocrinology and Metabolism·Aura D Herrera-MartínezRaúl M Luque
Dec 12, 2018·Minerva endocrinologica·Manuel Puig-Domingo, Monica Marazuela
Mar 13, 2019·The Journal of Clinical Endocrinology and Metabolism·Mari C Vázquez-BorregoRaúl M Luque
Jul 15, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel Hormaechea-AgullaRaúl M Luque
Dec 22, 2017·Journal of Cellular and Molecular Medicine·Eva Venegas-MorenoAlfonso Soto-Moreno
Jun 6, 2020·Journal of Clinical Medicine·Prudencio Sáez-MartínezRaúl M Luque
May 30, 2019·Frontiers in Endocrinology·Blake M HauserIan F Dunn
Oct 19, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mari C Vázquez-BorregoRaúl M Luque
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Jul 14, 2021·The Journal of Clinical Endocrinology and Metabolism·Juan M Jiménez-VacasRaúl M Luque
Aug 28, 2021·Diagnostics·Irene Alexandra SpiridonIrina Draga Căruntu
Jan 16, 2020·Neuroendocrinology·Mari C Vázquez-BorregoRaúl M Luque

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Datasets Mentioned

BETA
MDA-MB-231
U60179
U60181

Methods Mentioned

BETA
acylation
PCR
transfection
ELISAs
flow cytometry

Software Mentioned

SPSS
CLONTECH
GeNorm
GraphPad Prism5
ImageJ
MetaFluor
GraphPad Prism

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