Inaccuracy of routine creatinine measurement in canine urine

Veterinary Clinical Pathology
C TrumelJean-Pierre Braun

Abstract

Urine creatinine concentration often is used in ratios such as urine protein:creatinine to compensate for dilution or concentration of spot urine samples. The purpose of this study was to compare the accuracy of different techniques of urine creatinine measurement currently available for veterinary practitioners. In 104 samples of canine urine diluted 1:20 with distilled water, creatinine concentration was measured using a kinetic Jaffé reaction assay, and an enzymatic technique on an automatic analyzer (Elimat) and 3 benchtop analyzers (Reflovet, Scil; Vitros DT2, Ortho-Clinical Diagnostics; Vettest 8008, IDEXX) used in veterinary practice. The Jaffé and enzymatic techniques on the Elimat were not significantly different, and their inaccuracy tested with human control urines was <5%. The benchtop analyzers underestimated creatinine concentration, especially at concentrations >2000 mg/L. Inaccuracy was higher with multilayer slide technology systems (Vitros and Vettest) than with the Reflovet system. Results were approximately 25% and 2% lower, respectively, than with the Elimat at urine creatinine concentrations about 2000 mg/L. Inaccuracy in urine creatinine measurements using benchtop analyzers should be taken into account w...Continue Reading

References

Jul 1, 1997·Research in Veterinary Science·M UechiM Takahashi
Feb 5, 2003·Annals of Clinical Biochemistry·Nigel LawsonChristine Marenah

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Citations

Aug 27, 2016·Veterinary Clinical Pathology·Gabriele RossiSaverio Paltrinieri
Apr 13, 2005·Clinical Techniques in Small Animal Practice·Marie E Kerl, Cristi R Cook
Mar 28, 2008·Veterinary Clinical Pathology·Hervé P LefebvreJean-Pierre Braun
Oct 16, 2019·The Journal of Veterinary Medical Science·Keiichiro MieHideo Akiyoshi
Nov 4, 2020·Animals : an Open Access Journal From MDPI·Urszula PasławskaRobert Pasławski
Feb 2, 2021·Journal of Veterinary Science·Sumin JiYongbaek Kim
Jul 30, 2021·Veterinary Clinical Pathology·Marco GiraldiPaola Scarpa
Dec 21, 2007·Journal of Agricultural and Food Chemistry·Eun-Kee ParkBruce D Hammock

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