Inactivating BK channels in rat chromaffin cells may arise from heteromultimeric assembly of distinct inactivation-competent and noninactivating subunits

Biophysical Journal
J P DingC Lingle

Abstract

Inactivating and noninactivating variants of large-conductance, Ca2+-dependent, voltage-dependent BK-type channels are found in rat chromaffin cells and are largely segregated into different cells. Here we test the hypothesis that, within the population of cells that express inactivating BK current (BKi current), the BKi channels are largely heteromultimers composed of inactivation-competent subunits (bk(i)) and noninactivating subunits (bk(s)). Several independent types of evidence support this view. The gradual removal of inactivation by trypsin is consistent with the idea that in most cells and patches there are, on average, about two to three inactivation domains per channel. In addition, several aspects of blockade of BKi current by charybdotoxin (CTX) are consistent with the idea that BKi channels contain differing numbers (one to four) of relatively CTX-resistant bk(i) subunits. Finally, the frequency of occurrence of noninactivating BKs channels in patches with predominantly inactivating BKi channels is consistent with the binomial expectations of random, independent assembly of two distinct subunits, if most cells have, on average, about two to three bk(i) subunits per channel. These results suggest that the phenotypic...Continue Reading

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Citations

Apr 17, 2008·Pflügers Archiv : European journal of physiology·Rebecca PiskorowskiEllen A Lumpkin
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