PMID: 9531490Jun 11, 1998Paper

Inactivating peptide of the Shaker B potassium channel: conformational preferences inferred from studies on simple model systems

The Biochemical Journal
J A EncinarJ M Gonzalez-Ros

Abstract

Previous studies on the interaction between the inactivating peptide of the Shaker B K+ channel (ShB peptide, H2N-MAAVAGLYGLGEDRQHRKKQ) and anionic phospholipid vesicles, used as model targets, have shown that the ShB peptide: (i) binds to the vesicle surface with high affinity; (ii) readily adopts a strongly hydrogen-bonded beta-structure; and (iii) becomes inserted into the hydrophobic bilayer. We now report fluorescence studies showing that the vesicle-inserted ShB peptide is in a monomeric form and, therefore, the observed beta-structure must be intramolecularly hydrogen-bonded to produce a beta-hairpin conformation. Also, additional freeze-fracture and accessibility-to-trypsin studies, which aimed to estimate how deeply and in which orientation the folded monomeric peptide inserts into the model target, have allowed us to build structural models for the target-inserted peptide. In such models, the peptide has been folded near G6 to configure a long beta-hairpin modelled to produce an internal cancellation of net charges in the stretch comprising amino acids 1-16. As to the positively charged C-terminal portion of the ShB peptide (RKKQ), this has been modelled to be in parallel with the anionic membrane surface to facilitat...Continue Reading

Citations

Oct 15, 2011·Biochimica Et Biophysica Acta·Zhuo FanZhongju Xiao
Dec 1, 2012·Biochimica Et Biophysica Acta·Zhuo FanZhongju Xiao

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