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Inactivation and augmentation of the primary 3-ketosteroid-{delta}1- dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene- 3,17-dione or 1,4-androstadiene-3,17-dione

Applied and Environmental Microbiology

May 11, 2010

Wei WeiDong-Zhi Wei

Abstract

3-Ketosteroid-Delta(1)-dehydrogenase, KsdD(M), was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separating 4-androstene-3,17-dione (AD) from 1,4-androstadiene-3,17-dione (ADD) is a key bottleneck ...read more

Mentioned in this Paper

1,4-androstadiene-3,17-dione
Dione
Androstenes
Ketosteroids
Glycine max
Soybean preparation
Oxidase
Gene Deletion
Genetic Manipulation
Phytosterols
29
Paper Details
References
  • References17
  • Citations23
12
  • References17
  • Citations23
123

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Bioinformatics in Biomedicine

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Inactivation and augmentation of the primary 3-ketosteroid-{delta}1- dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene- 3,17-dione or 1,4-androstadiene-3,17-dione

Applied and Environmental Microbiology

May 11, 2010

Wei WeiDong-Zhi Wei

PMID: 20453136

DOI: 10.1128/aem.00448-10

Abstract

3-Ketosteroid-Delta(1)-dehydrogenase, KsdD(M), was identified by targeted gene disruption and augmentation from Mycobacterium neoaurum NwIB-01, a newly isolated strain. The difficulty of separating 4-androstene-3,17-dione (AD) from 1,4-androstadiene-3,17-dione (ADD) is a key bottleneck ...read more

Mentioned in this Paper

1,4-androstadiene-3,17-dione
Dione
Androstenes
Ketosteroids
Glycine max
Soybean preparation
Oxidase
Gene Deletion
Genetic Manipulation
Phytosterols
29

Similar Papers Found In These Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Paper Details
References
  • References17
  • Citations23
12
  • References17
  • Citations23
123
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