PMID: 8968954Oct 1, 1996Paper

Inactivation and conformational changes of aminoacyclase in trifluoroethanol solutions

Journal of Protein Chemistry
Y X ZhangH M Zhou

Abstract

The inactivation and unfolding of aminoacyclase (EC 3.5.1.14) during denaturation by different concentrations of trifluoroethanol (TFE) have been studied. A marked decrease in enzyme activity was observed at low TFE concentrations. The kinetic theory of the substrate reaction during irreversible inhibition of enzyme activity described previously by Tsou [Tsou (1988), Adv. Enzymol. Related Areas Mol. Biol. 61, 381-436] was applied to study the kinetics of the inactivation course of aminoacyclase during denaturation by TFE. The inactivation rate constants for the free enzyme and substrate-enzyme complex were determined by Tsou's method. The inactivation reaction was a monophasic first-order reaction. The kinetics of the unfolding course were a biphasic process consisting of two first-order reactions. At 2% TFE concentration, the inactivation rate of the enzyme was much faster than the unfolding rate. At a higher concentration of TFE (10%), the inactivation rate was too fast to be determined by conventional methods, whereas the unfolding course remained as a biphasic process with fast and slow reactions occurring at measurable rates. The results suggest that the aminoacyclase active site containing Zn2+ ions is situated in a limit...Continue Reading

References

Sep 14, 1976·Biochimica Et Biophysica Acta·W Kördel, F Schneider
May 1, 1977·Zeitschrift Für Naturforschung. Section C: Biosciences·W Kördel, F Schneider
Jun 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W Kördel, F Schneider
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Mar 1, 1994·Biochemistry·D V Waterhous, W C Johnson

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Citations

Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Qiang Xie, Hai-Meng Zhou
Aug 15, 2000·The International Journal of Biochemistry & Cell Biology·Y X ZhangH M Zhou
Jun 26, 2003·The International Journal of Biochemistry & Cell Biology·Qiang XieHai-Meng Zhou
Feb 8, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Sung-Hye KimHai-Meng Zhou
Oct 22, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xiao-Yun WangHai-Meng Zhou
Feb 6, 2014·Physical Chemistry Chemical Physics : PCCP·Srabanti GhoshSomobrata Acharya
Apr 26, 2002·The Journal of Biological Chemistry·Sunggoo YunHee Cheon Lee
Aug 29, 2006·Biochimica Et Biophysica Acta·Rakesh Mohan KestwalShobhana V Bhide

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