Inactivation and neutralization of parvovirus B19 Genotype 3

Transfusion
Johannes BlümelSally A Baylis

Abstract

Parvovirus B19 (B19V) is a common contaminant of human plasma donations. Three B19V genotypes have been defined based on their DNA sequence. Reliable detection of Genotype 3 DNA has proved problematic because of unexpected sequence variability. B19V Genotype 3 is found primarily in West Africa, but was recently detected in plasma from a North American donor. The safety of plasma-derived medicinal products, with respect to B19V, relies on exclusion of high-titer donations, combined with virus clearance at specific manufacturing steps. Studies on inactivation of B19V are difficult to perform and inactivation of Genotype 3 has not yet been investigated. Inactivation of B19V Genotypes 3 and 1 by pasteurization of human serum albumin and incubation at low pH was studied using a cell culture assay for infectious virus particles. Infected cells were detected by reverse transcription-polymerase chain reaction analysis of virus capsid mRNA. Neutralization of B19V Genotype 3 was investigated using human immunoglobulin preparations. Genotypes 1 and 3 displayed comparable inactivation kinetics during pasteurization of albumin at 56°C, as well as by incubation at various low-pH conditions (pH 4.2 at 37°C and pH 4.5 at 23°C, respectively) us...Continue Reading

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Citations

Dec 18, 2013·Biologicals : Journal of the International Association of Biological Standardization·Rong Ma, Xiaolan Cui
Sep 17, 2013·Transfusion·Sally A BaylisJohannes Blümel
Jul 31, 2016·Biologicals : Journal of the International Association of Biological Standardization·Raymond W Nims, S Steve Zhou
Feb 17, 2018·Frontiers in Medicine·David Juhl, Holger Hennig

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