PMID: 8590603Jul 1, 1995Paper

Inactivation and unfolding of aminoacylase during denaturation in sodium dodecyl sulfate solutions

Journal of Protein Chemistry
B HeH M Zhou

Abstract

During denaturation by sodium dodecyl sulfate (SDS), aminoacylase shows a rapid decrease in activity with increasing concentration of the detergent to reach complete inactivation at 1.0 mM SDS. The denatured minus native-enzyme difference spectrum showed two negative peaks at 287 and 295 nm. With the increase of concentration of SDS, both negative peaks increased in magnitude to reach maximal values at 5.0 mM SDS. The fluorescence emission intensity of the enzyme decreased, whereas there was no red shift of emission maximum in SDS solutions of increasing concentration. In the SDS concentration regions employed in the present study, no marked changes of secondary structure of the enzyme have been observed by following the changes in far-ultraviolet CD spectra. The inactivation of this enzyme has been followed and compared with the unfolding observed during denaturation in SDS solutions. A marked inactivation is already evident at low SDS concentration before significant conformational changes can be detected by ultraviolet absorbance and fluorescence changes. The inactivation rate constants of free enzyme and substrate-enzyme complex were determined by the kinetics method of the substrate reaction in the presence of inactivator ...Continue Reading

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Citations

Aug 30, 2011·European Biophysics Journal : EBJ·Huei-Fen LoLong-Liu Lin
Nov 19, 2009·The Protein Journal·Xue-Qiang WuXiao-Yun Wang
Dec 14, 2001·Journal of Biochemical and Biophysical Methods·Eduardo LissiCarlos Alvarez
Aug 15, 2000·The International Journal of Biochemistry & Cell Biology·G F WangY F Zhao
Jul 11, 2006·International Journal of Biological Macromolecules·Sung-Hye KimYong-Bin Yan
Jan 31, 2012·International Journal of Biological Macromolecules·Tzu-Ting ChuangLong-Liu Lin
Dec 17, 2002·The Journal of Biological Chemistry·Luis J del VallePere Garriga
Jun 1, 1996·International Journal of Peptide and Protein Research·W P LeH M Zhou
Aug 1, 1996·International Journal of Biological Macromolecules·T Zhang, H M Zhou
Aug 24, 2001·International Journal of Biological Macromolecules·M BozziM Paci

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