Inactivation Efficacy of Nonthermal Plasma-Activated Solutions against Newcastle Disease Virus

Applied and Environmental Microbiology
Xia SuJing Fang

Abstract

In recent years, plasma-activated solutions (PASs) have made good progress in the disinfection of medical devices, tooth whitening, and fruit preservation. In this study, we investigated the inactivation efficacy of Newcastle disease virus by PASs. Water, 0.9% NaCl, and 0.3% H2O2 were excited by plasma to obtain the corresponding solutions PAS(H2O), PAS(NaCl), and PAS(H2O2). The complete inactivation of virus after PAS treatment for 30 min was confirmed by the embryo lethality assay (ELA) and hemagglutination (HA) test. Scanning electron microscopy (SEM) results showed that the morphology of the viral particle changed under PAS treatments. The total protein concentration of virus decreased as measured by a Bradford protein assay due to PAS treatment. The nucleic acid integrity assay demonstrated that viral RNA degraded into smaller fragments. Moreover, the physicochemical properties of PASs, including the oxidation-reduction potential (ORP), electrical conductivity, and H2O2 concentration, and electron spin resonance spectra analysis indicated that reactive oxygen and nitrogen species play a major role in the virus inactivation. Therefore, the application of PASs, as an environmentally friendly method, would be a promising alte...Continue Reading

References

Jan 1, 1994·Methods in Molecular Biology·N J Kruger
Jul 1, 1993·British Medical Bulletin·K H Cheeseman, T F Slater
Mar 16, 2000·Developmental and Comparative Immunology·B S SealH S Sellers
Aug 10, 2000·Revue Scientifique Et Technique·D J Alexander
Sep 12, 2000·The Journal of Antimicrobial Chemotherapy·M TagawaH Saisho
Mar 7, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ching-You LuYau-Huei Wei
Jun 6, 2003·Journal of the American Veterinary Medical Association·David E Swayne, Daniel J King
Jan 28, 2009·Letters in Applied Microbiology·G Kamgang-YoubiM Naïtali
Oct 5, 2010·Applied and Environmental Microbiology·Murielle NaïtaliJean-Louis Brisset
May 10, 2011·American Journal of Infection Control·Sagar M GoyalPeter C Raynor
Dec 14, 2011·Annual Review of Food Science and Technology·Brendan A Niemira
Apr 30, 2013·Developmental and Comparative Immunology·Darrell R KapczynskiPatti J Miller
Mar 5, 2014·Virus Research·Ketan GanarSachin Kumar
Apr 24, 2014·Translational Vision Science & Technology·Oleg AlekseevJane Azizkhan-Clifford
Nov 25, 2014·Applied and Environmental Microbiology·Yan WuSergey A Grinshpun
Mar 22, 2015·Applied and Environmental Microbiology·Hamada A AboubakrSagar M Goyal
Aug 19, 2015·Journal of Hazardous Materials·Ruonan MaJing Fang
Nov 4, 2015·Vaccine·Guomin WangJing Fang

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Citations

Jun 20, 2018·Applied and Environmental Microbiology·Li GuoMichael G Kong
Jul 26, 2018·Biological Chemistry·Nagendra Kumar KaushikEun Ha Choi
Sep 2, 2020·Emerging Microbes & Infections·Qiang ZhangMeilin Jin
May 16, 2019·Applied Microbiology and Biotechnology·Juliana ŠimončicováBarbora Kaliňáková
Dec 3, 2020·Critical Reviews in Food Science and Nutrition·Qisen XiangYanhong Bai
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Sellam PerinbanVijaya Raghavan
Mar 16, 2021·Journal of Pharmaceutical Analysis·Lingge GaoXili Wu
Aug 21, 2021·Journal of Hazardous Materials·Kaige SongFei Ling
Feb 8, 2021·Transboundary and Emerging Diseases·Ya ZhaoMeilin Jin

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Methods Mentioned

BETA
electron spin resonance
electrophoresis
chip
Fluorescence
ESR
protein assay
chips

Software Mentioned

Bioanalyzer
SPSS
Agilent Biosizing

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