Inactivation of A. ochraceus spores and detoxification of ochratoxin A in coffee beans by gamma irradiation

Journal of Food Science
Sanjeev KumarArun Sharma

Abstract

Ochratoxin A (OTA) produced in food by Aspergillus ochraceus is known to cause adverse health effects. Among the plantation products, green coffee beans are prone to fungal attack and get contaminated with OTA frequently. A fungal strain isolated from green coffee beans was characterized by morphological analyses as well as internal transcribed spacer (ITS) and 5.8S rDNA sequencing, turned out to be A. ochraceus, however, nontoxigenic. Hence, additional strains of A. ochraceus were procured and characterized for toxin production. Presterilized green coffee beans were spiked with a toxigenic strain and treated with gamma radiation. Minimum inhibitory dose (MID) of gamma radiation for 10(4) and 10(8) spores of A. ochraceus strain per 10 g of green coffee beans was found to be approximately 1 and approximately 2.5 kGy, respectively. The radiation treatment (10 kGy) almost degraded the preformed or in vitro added OTA (50 ppb) in coffee beans. OTA degradation was found to be enhanced with increase in moisture content. Cytotoxicity in terms of cell viability was found to be reduced significantly for radiation treated OTA in MTT [3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide] assay as well as flow cytometric analysis w...Continue Reading

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Citations

Dec 3, 2013·Current Microbiology·Kun-hon LeongFujitoshi Yanagida
May 12, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ana-Marija DomijanKsenija Markov
Nov 1, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Lijun QiZhengxing Chen
Apr 28, 2018·Journal of the Science of Food and Agriculture·Bang-Yuan ChenChung-Yi Wang
Mar 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eliana PereiraPaula Rodrigues
Jan 8, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ana-Marija DomijanBranka Mihaljević
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Yue LiuCaroline Orfila
May 8, 2021·Toxicon : Official Journal of the International Society on Toxinology·Ola A A KhalilAhmed Salem Sebaei

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