Abstract
Rapid and effective virus inactivation is an essential step for safe diagnostic testing and for research and vaccine development using infectious viruses. We characterised the reduction of African Swine Fever Virus (ASFV) infectivity using Virkon™ S (Lanxess) 1% w/v disinfectant, FACS™ Lysing buffer (BD), and AVL™ buffer (Qiagen), using porcine cell culture. No virus was detected following a 30 s 20:1 v/v mixing ratio of Virkon™ S 1% with high titre ASFV, supporting its effective use as a laboratory surface disinfectant. FACS™ Lysing and AVL™ buffers also inactivated ASFV, permitting safe removal of treated infected samples from high containment facilities.
References
Apr 2, 1993·Journal of Immunological Methods·J K NicholsonJ S McDougal
Aug 5, 1999·Journal of Applied Microbiology·C Turner, S M Williams
Feb 17, 2000·The Journal of Veterinary Medical Science·J ShiraiR Seki
Sep 29, 2004·Antiviral Research·Lisa McCormick, Gargi Maheshwari
May 30, 2009·Diseases of Aquatic Organisms·Laura K BryanDebra L Miller
Jul 30, 2011·Biologicals : Journal of the International Association of Biological Standardization·Peter W KrugLuis Rodriguez
May 23, 2012·Emerging Infectious Diseases·Adriana V DiazAnthony J Wilson
Oct 9, 2012·Virus Research·Patricia de LeónAngel L Carrascosa
Jan 1, 2013·Virus Research·A S OganesyanS A Dudnikov
Dec 3, 2014·Journal of Comparative Pathology·J M Sánchez-VizcaínoL Carrasco
Nov 23, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Curt I CivinJames C Sturm
Feb 9, 2017·Journal of Virological Methods·Guanghui WuAshley Banyard
Oct 11, 2017·Vaccines·Marisa AriasUNKNOWN Sanchez-Vizcaino
Oct 28, 2018·Infectious Diseases of Poverty·Tao WangHua-Ji Qiu
Apr 17, 2019·Journal of Veterinary Research·Małgorzata JuszkiewiczGrzegorz Woźniakowski
Oct 2, 2019·Journal of Veterinary Research·Natalia Mazur-PanasiukGrzegorz Woźniakowski
Feb 9, 2020·Journal of Virological Methods·Britta A WoodMichael Eschbaumer
May 8, 2020·Journal of Applied Microbiology·Lindsay R GabbertMax Rasmussen
Oct 30, 2020·Pathogens·Małgorzata JuszkiewiczGrzegorz Woźniakowski