Inactivation of bpsl1039-1040 ATP-binding cassette transporter reduces intracellular survival in macrophages, biofilm formation and virulence in the murine model of Burkholderia pseudomallei infection

PloS One
Peechanika PinwehaSunee Korbsrisate

Abstract

Burkholderia pseudomallei, a gram-negative intracellular bacillus, is the causative agent of a tropical infectious disease called melioidosis. Bacterial ATP-binding cassette (ABC) transporters import and export a variety of molecules across bacterial cell membranes. At present, their significance in B. pseudomallei pathogenesis is poorly understood. We report here characterization of the BPSL1039-1040 ABC transporter. B. pseudomallei cultured in M9 medium supplemented with nitrate, demonstrated that BPSL1039-1040 is involved in nitrate transport for B. pseudomallei growth under anaerobic, but not aerobic conditions, suggesting that BPSL1039-1040 is functional under reduced oxygen tension. In addition, a nitrate reduction assay supported the function of BPSL1039-1040 as nitrate importer. A bpsl1039-1040 deficient mutant showed reduced biofilm formation as compared with the wild-type strain (P = 0.027) when cultured in LB medium supplemented with nitrate under anaerobic growth conditions. This reduction was not noticeable under aerobic conditions. This suggests that a gradient in oxygen levels could regulate the function of BPSL1039-1040 in B. pseudomallei nitrate metabolism. Furthermore, the B. pseudomallei bpsl1039-1040 mutant ...Continue Reading

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Citations

Mar 14, 2020·Biochemical Society Transactions·Chee-Hoo YipSheila Nathan
Feb 19, 2020·Pathogens and Disease·Joseph D ThiriotAlfredo G Torres
Feb 8, 2021·Experimental Eye Research·Konduri RanjithSisinthy Shivaji
Mar 19, 2021·Saudi Journal of Biological Sciences·Madiha ZaynabShuangfei Li

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Methods Mentioned

BETA
PCR

Software Mentioned

HHsearch
Poing
Discovery Studio Visualizer
GraphPad
GraphPad Prism
CLC Drug Discovery Workbench
Accelrys

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