Inactivation of Burkholderia cepacia complex phage KS9 gp41 identifies the phage repressor and generates lytic virions.

Journal of Virology
Karlene H LynchJonathan J Dennis

Abstract

The Burkholderia cepacia complex (BCC) is made up of at least 17 species of gram-negative opportunistic bacterial pathogens that cause fatal infections in patients with cystic fibrosis and chronic granulomatous disease. KS9 (vB_BcenS_KS9), one of a number of temperate phages isolated from BCC species, is a prophage of Burkholderia pyrrocinia LMG 21824. Transmission electron micrographs indicate that KS9 belongs to the family Siphoviridae and exhibits the B1 morphotype. The 39,896-bp KS9 genome, comprised of 50 predicted genes, integrates into the 3' end of the LMG 21824 GTP cyclohydrolase II open reading frame. The KS9 genome is most similar to uncharacterized prophage elements in the genome of B. cenocepacia PC184 (vB_BcenZ_ PC184), as well as Burkholderia thailandensis phage phiE125 and Burkholderia pseudomallei phage phi1026b. Using molecular techniques, we have disrupted KS9 gene 41, which exhibits similarity to genes encoding phage repressors, producing a lytic mutant named KS9c. This phage is incapable of stable lysogeny in either LMG 21824 or B. cenocepacia strain K56-2 and rescues a Galleria mellonella infection model from experimental B. cenocepacia K56-2 infections at relatively low multiplicities of infection. These ...Continue Reading

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Citations

Apr 20, 2013·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Susan HoeReinhard Vehring
Dec 6, 2011·Applied and Environmental Microbiology·Karlene H LynchJonathan J Dennis
Feb 22, 2012·Canadian Journal of Microbiology·Karlene H Lynch, Jonathan J Dennis
Oct 27, 2010·BMC Genomics·Karlene H LynchJonathan J Dennis
Jun 21, 2016·Journal of Applied Microbiology·P BárdyJ Doškař
Dec 3, 2014·Applied and Environmental Microbiology·Fatima Kamal, Jonathan J Dennis
Apr 22, 2021·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Małgorzata ŁobockaAndrzej Górski
Aug 11, 2021·Viruses·Philip Lauman, Jonathan J Dennis

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