Inactivation of CaMIT1 inhibits Candida albicans phospholipomannan beta-mannosylation, reduces virulence, and alters cell wall protein beta-mannosylation

The Journal of Biological Chemistry
Céline MilleD Poulain

Abstract

Studies on Candida albicans phospholipomannan have suggested a novel biosynthetic pathway for yeast glycosphingolipids. This pathway is thought to diverge from the usual pathway at the mannose-inositol-phospho-ceramide (MIPC) step. To confirm this hypothesis, a C. albicans gene homologue for the Saccharomyces cerevisiae SUR1 gene was identified and named MIT1 as it coded for GDP-mannose:inositol-phospho-ceramide mannose transferase. Two copies of this gene were disrupted. Western blots of cell extracts revealed that strain mit1Delta contained no PLM. Thin layer chromatography and mass spectrometry confirmed that mit1Delta did not synthesize MIPC, demonstrating a role of MIT1 in the mannosylation of C. albicans IPCs. As MIT1 disruption prevented downstream beta-1,2 mannosylation, mit1Delta represents a new C. albicans mutant affected in the expression of these specific virulence attributes, which act as adhesins/immunomodulators. mit1Delta was less virulent during both the acute and chronic phases of systemic infection in mice (75 and 50% reduction in mortality, respectively). In vitro, mit1Delta was not able to escape macrophage lysis through down-regulation of the ERK1/2 phosphorylation pathway previously shown to be triggered...Continue Reading

References

Sep 1, 1992·Infection and Immunity·P A TrinelD Poulain
Dec 1, 1969·Journal of Bacteriology·J Kocourek, C E Ballou
Jul 1, 1996·Journal of Leukocyte Biology·C FradinD Poulain
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·C A Wiggins, S Munro
Jul 14, 2001·Nature·M C Lorenz, G R Fink
Jul 26, 2002·The Journal of Biological Chemistry·Pierre-André TrinelDaniel Poulain
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Françoise DromerDaniel Poulain
Mar 20, 2003·The Journal of Biological Chemistry·Arnaud LagorceJean François
Jun 26, 2003·The Journal of Infectious Diseases·Thierry JouaultDaniel Poulain
Jul 28, 1951·Nature·M DUBOISF SMITH
Jul 28, 1951·Nature·F BRYANT, B T OVERELL
Sep 11, 2004·Current Opinion in Microbiology·Daniel Poulain, Thierry Jouault

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Citations

Feb 5, 2013·The Journal of Antimicrobial Chemotherapy·Ute ZeidlerChristophe d'Enfert
Dec 22, 2005·Infection and Immunity·Lena J HeungMaurizio Del Poeta
Jun 26, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Nobuyuki ShibataShigeo Suzuki
Apr 15, 2014·Lasers in Medical Science·Rodnei Dennis RossoniJuliana Campos Junqueira
Mar 31, 2016·Nature·David L MoyesJulian R Naglik
Aug 22, 2013·Critical Reviews in Microbiology·Daniel Poulain
May 24, 2015·FEMS Yeast Research·Richard CalderoneAna Traven
Nov 15, 2014·Seminars in Immunopathology·Chantal FradinThierry Jouault
Jan 21, 2006·FEMS Yeast Research·José Ruiz-HerreraRafael Sentandreu
May 28, 2005·Yeast
Oct 16, 2013·Molecular Microbiology·Rebecca A Hall, Neil A R Gow
Mar 13, 2020·Biological & Pharmaceutical Bulletin·Chiho YanaiYoshiyuki Adachi
Jul 17, 2020·Expert Review of Anti-infective Therapy·Caroline Mota Fernandes, Maurizio Del Poeta
Sep 8, 2018·Frontiers in Microbiology·Hassan O J MoradChristopher R Thornton
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine A WellsRobert B Ashman
Jan 30, 2019·Frontiers in Microbiology·João Henrique Tadini Marilhano FabriIran Malavazi
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thierry JouaultDaniel Poulain
May 20, 2020·The Journal of Organic Chemistry·Veeranjaneyulu GannediRam A Vishwakarma

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