Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
L LichkoI Kulaev

Abstract

Saccharomyces cerevisiae possesses multiple forms of exopolyphosphatases, the enzymes involved in the metabolism of inorganic polyphosphates, which are important regulatory compounds. In S. cerevisiae, inactivation of endopolyphosphatase gene PPN1 leads to the inhibition of expression of both exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase of approximately 1000 kDa not encoded by PPX1. In the single endopolyphosphatase mutant CRN, the expression of exopolyphosphatase PPX1 decreases 6.5-fold and 2.5-fold at the stationary and exponential growth phases, respectively, as compared with the parent strain CRY. In this mutant, the activity of the high-molecular-mass exopolyphosphatase of approximately 1000 kDa decreases approximately 10-fold as compared with that in strains with the PPN1 gene. In a double mutant of PPX1 and PPN1, no exopolyphosphatase activity is detected in the cytosol at the stationary growth phase. Thus, the exopolyPase activity in cell cytosol depends on the endopolyPase gene PPN1.

References

Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J D KeaslingA Kornberg
Jul 31, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·D ChatterjiA Ishihama
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Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·A SethuramanA Kornberg
Apr 12, 2003·FEMS Yeast Research·Lidia P LichkoIgor S Kulaev

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Citations

Oct 13, 2009·Biochemistry. Biokhimii︠a︡·L P LichkoI S Kulaev
Feb 15, 2011·Biochemistry. Biokhimii︠a︡·L P LichkoI S Kulaev
Sep 29, 2011·Clinical Biochemistry·D S Robertson
Mar 15, 2005·Yeast
Feb 21, 2019·FEMS Yeast Research·A Ya ValiakhmetovA O Shepelyakovskaya
Apr 9, 2021·Biochemistry. Biokhimii︠a︡·Tatiana V KulakovskayaMichail A Eldarov

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