Inactivation of Escherichia coli O157:H7 on inoculated alfalfa seeds with ozonated water and heat treatment

Journal of Food Protection
Ratna R SharmaW F Fett

Abstract

Alfalfa seeds inoculated with a five-strain mixture of Escherichia coli O157:H7 were immersed in water containing 4, 8, 10, and 21 ppm of ozone for 2, 4, 8, 16, 32, and 64 min at 4 degrees C. Direct ozone sparging of seeds in water was used as an alternative mode of ozone treatment. Ozone-sparged seeds were also subsequently exposed to heat treatment at 40, 50, and 60 degrees C for 3 h. Populations of E. coli O157:H7 on untreated and treated seeds were determined by spread plating diluted samples on tryptic soy agar supplemented with 50 microg/ml of nalidixic acid. Since E. coli O157:H7 was released from inoculated seeds during treatment with ozone, the rate of release of cells from inoculated seeds soaked in 0.1% peptone water for up to 64 min was also determined. The overall reduction of E. coli O157:H7 on seeds treated with ozonated water without continuous sparging ranged from 0.40 to 1.75 log10 CFU/g (59.6 to 98.2%), whereas reductions for control seeds were 0.32 to 1.03 log10 CFU/g (51.7 to 90.5%). Treatment with higher ozone concentrations enhanced inactivation, but contact time longer than 8 min did not result in significantly higher reductions (P > 0.05). For seeds treated by ozone sparging, a 1.12-log10 CFU/g (92.1%) ...Continue Reading

Citations

Apr 16, 2004·Journal of Food Protection·Rebecca Montville, Donald W Schaffner
Feb 5, 2005·Applied and Environmental Microbiology·Rebecca Montville, Donald Schaffner
Jul 15, 2005·Journal of Food Protection·Joseph M BosilevacMohammad Koohmaraie
Jan 19, 2006·Journal of Food Protection·Shigenobu Koseki, Seiichiro Isobe
Apr 16, 2004·Journal of Food Protection·Kathleen T Rajkowski, Eugene W Rice
Dec 2, 2011·Annual Review of Food Science and Technology·Jennifer J Perry, Ahmed E Yousef
Jun 13, 2003·Journal of Food Protection·Elke Y WuytackChris W Michiels
Jul 24, 2014·Foodborne Pathogens and Disease·Qing Wang, Kalmia E Kniel
Jul 16, 2011·International Journal of Analytical Chemistry·Vildes Maria ScusselManuel Nazaré Ferreira Rodrigues
Sep 11, 2018·International Journal of Food Microbiology·Zahra MohammadAlejandro Castillo
Apr 8, 2021·European Food Research and Technology = Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung. a·Duygu Alp, Özcan Bulantekin

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