Inactivation of glutaraldehyde by reaction with sodium bisulfite

Journal of Toxicology and Environmental Health
S L JordanA B Theis

Abstract

The microbiocidal activity of glutaraldehyde was inactivated by reaction with sodium bisulfite via formation of a proposed glutaraldehyde-bisulfite complex. High-performance liquid chromatography (HPLC) analysis of 2% (0.2M) alkaline glutaraldehyde indicated complete loss of glutaraldehyde at a 2.2:1 molar ratio of sodium bisulfite to glutaraldehyde. Neither 1.7% (0.17 M) sodium bisulfite alone nor the glutaraldehyde-bisulfite complex was microbiocidal when tested against Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, and Polybac Polyseed BOD seed inoculum. Bacterial inhibition tests indicated that the glutaraldehyde-sodium bisulfite complex had no effect on the growth of sewage microorganisms at concentrations as high as 50-100 ppm (5 x 10(-4)-1 x 10(-3) M), with an IC50 of 230-440 ppm (2.3 x 10(-3)-4.4 x 10(-3) M), based on glutaraldehyde concentration. A 28-close bottle test showed a 5-d biodegradation of 48% and 51%, and a 15-d biodegradation of 57% and 63% for 3:1 and 2.2:1 bisulfite to glutaraldehyde molar ratios, respectively. Acute aquatic toxicity testing with Daphnia magna demonstrated an LC50 of 41-109 ppm (4.1 x 10(-4)-10.9 x 10(-4) M) and a no-observed-effect concentration (NOEC) of 16 ppm (1.6 x...Continue Reading

Citations

Oct 27, 2012·Journal of Microencapsulation·Naveen K BejugamMartin J D'Souza
Jul 3, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Neeraj MishraSuresh P Vyas
Oct 18, 2013·The Science of the Total Environment·Susana P P PereiraAntónio J A Nogueira
Feb 19, 2013·Journal of Agricultural and Food Chemistry·Zi TengQin Wang
Jun 2, 2001·Ecotoxicology and Environmental Safety·H W Leung
Oct 3, 2018·Future Science OA·Roshini Prakash, Stanley Thomas Carmichael
Jul 3, 2021·Pharmaceutics·Mohammad Mirazul IslamMiguel González-Andrades

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