PMID: 9540854Apr 16, 1998Paper

Inactivation of glyceraldehyde 3-phosphate dehydrogenase by sugars, prednisolone-21-hemisuccinate, cyanate and other small molecules

Biochimica Et Biophysica Acta
D W Hook, J J Harding

Abstract

Diabetes, diarrhoea, renal failure and glucocorticoid therapy have all been identified as independent risk factors for cataract. Increased post-translational modification of proteins, leading to inactivation of enzymes and induction of conformational changes within proteins could result in lens opacification and cataract. Aspirin has been associated with many beneficial effects, including protection against cataract, in-vivo. alpha-Crystallin has been shown to act as a molecular chaperone in-vitro. This lenticular protein prevented the thermal aggregation of other lens proteins in-vitro and has sequence and functional homology with the small heat shock proteins. Glyceraldehyde 3-phosphate dehydrogenase (GAP-DH) is constitutively expressed in tissues and is susceptible to chemical modification in-vivo. In-vitro incubations of GAP-DH with sugars, cyanate and prednisolone-21-hemisuccinate, all led to significant loss of enzyme activity with time in two buffer systems. Rapid inactivation occurred when GAP-DH was incubated with fructose 6-phosphate or prednisolone-21-hemisuccinate. Slower inactivation was observed when GAP-DH was incubated with fructose, glucose 6-phosphate or potassium cyanate. Glucose did not inactivate GAP-DH und...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Horwitz
Nov 15, 1992·The Biochemical Journal·R Blakytny, J J Harding
Apr 1, 1992·Experimental Eye Research·R Blakytny, J J Harding
Jan 26, 1990·Science·B S SzwergoldT R Brown
Feb 1, 1990·Experimental Eye Research·K A Roberts, J J Harding
Jan 16, 1989·Biochemical and Biophysical Research Communications·S P Bhat, C N Nagineni
Mar 1, 1988·Experimental Eye Research·M OimomiM Yamamoto
Feb 13, 1986·The New England Journal of Medicine·V M MonnierR R Kohn
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T D Ingolia, E A Craig
Nov 1, 1984·The Journal of Clinical Investigation·S ManabeA Cerami
Aug 1, 1983·Experimental Eye Research·J Jedziniak, J Rokita
Jul 1, 1995·Journal of the American Society of Nephrology : JASN·A ConjardG Baverel
Apr 26, 1995·Biochemical and Biophysical Research Communications·J Jimenez-AsensioD L Garland
Nov 11, 1993·International Journal of Cancer. Journal International Du Cancer·A AoyamaR Klemenz
Nov 1, 1995·Biochemical Society Transactions·R Blakytny, J J Harding

❮ Previous
Next ❯

Citations

Apr 26, 2011·Molecular Biotechnology·Ashok D ChougaleMahesh J Kulkarni
Jun 9, 2004·Experimental Eye Research·Diyaa RachdanJohn J Harding
Apr 27, 1999·Progress in Retinal and Eye Research·B K Derham, J J Harding
Jan 22, 2000·The Biochemical Journal·E Ganea, J J Harding
Sep 4, 2010·Antioxidants & Redox Signaling·Stefano M Marino, Vadim N Gladyshev
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Arthur Stevens
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Andrew I Jobling, Robert C Augusteyn
Jan 16, 2003·Biochimica Et Biophysica Acta·Paul J BeisswengerBenjamin S Szwergold
Jun 16, 2005·Biochimica Et Biophysica Acta·Hong Yan, John J Harding
May 3, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Anna Maria BassiDamiano Cottalasso
Sep 25, 2017·Biochemistry. Biokhimii︠a︡·V I MuronetzE V Schmalhausen
Jun 25, 1999·Biochimica Et Biophysica Acta·H Yan, J J Harding

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Biological Macromolecules
D W Hook, J J Harding
Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde
J JagerA J Smit
© 2022 Meta ULC. All rights reserved