Inactivation of Gram (-) bacteria Salmonella enterica by chlorophyllin-based photosensitization: Mechanism of action and new strategies to enhance the inactivation efficiency

Journal of Photochemistry and Photobiology. B, Biology
Irina BuchovecZivile Luksiene

Abstract

This study is focused on the enhancement of susceptibility of Gram (-) bacteria S. enterica to chlorophyllin-based (Chl) photosensitization combining it with other antimicrobial tools. In order to find best combinations, the mechanism by which Chl-based photosensitization inactivates bacteria must be identified. Data confirmed that photosensitization (Chl 1.5×10(-5)M, for 1-120min, 405nm, 0-46.1J/cm(2)) reduced S. enterica population, just by 2.05 log (CFU/ml). Fluorimetric measurements indicated that just minor part of Chl was bound to Salmonella in suspension. Addition of sodium azide (NaN3) (10mM) protected bacteria from killing, what means that (1)O2 took place in photochemical reactions. Gene expression data confirmed that Chl-based photosensitization induced oxidative stress in bacteria cells, since mostly genes responsible for detoxification of ROS (OxyR, AhpC, GrxA) have been expressed in Salmonella. Moreover, the expression of genes, responsible for the inhibition of oxidative respiration (AtpC), cell division and down-regulation of metabolism (SulA) have been detected. In addition, Chl-based photosensitization induced significant release of intracellular components (absorbing at λ260 nm and λ280 nm) in bacteria that i...Continue Reading

Citations

Jun 22, 2018·World Journal of Microbiology & Biotechnology·Jun-Nan ZhangXiang-Hong Meng
Sep 5, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bernadeta Žudytė, Živilė Lukšienė
Jul 31, 2020·Journal of Biophotonics·Bernadeta ŽudytėŽivilė Lukšienė
Sep 25, 2020·International Journal of Molecular Sciences·Irina BuchovecPranciškus Vitta
Mar 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Vinayak S GhateHyun-Gyun Yuk
Jun 1, 2021·Food Research International·Marco CossuAndrea Cossu
Jun 1, 2021·Frontiers in Medicine·Aleksandra Rapacka-ZdończykJoanna Nakonieczna
Jul 9, 2021·Scientifica·Renny IndrawatiTatas Hardo Panintingjati Brotosudarmo
Aug 31, 2021·Critical Reviews in Food Science and Nutrition·Dan LiuJianxia Sun

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