Inactivation of hepatitis B virus and non-A, non-B hepatitis by chloroform.

Infection and Immunity
S M FeinstoneR H Purcell

Abstract

To determine whether a non-A, non-B hepatitis agent contained essential lipids, we extracted with chloroform a dilution of human plasma that contained approximately 10(4) chimpanzee infectious doses of non-A, non-B hepatitis virus and then tested for infectivity in chimpanzees. In addition, we treated a serum containing hepatitis B virus in the same way. Both of these samples were also sham extracted as controls. Known chloroform-sensitive and chloroform-resistant viruses were added directly to the hepatitis-containing serum or plasma as internal controls or to fetal calf serum as external controls and were assayed for infectivity in vitro after chloroform extraction or sham extraction. All infectivity of the diluted plasma that contained at least 10(4) chimpanzee infective doses of non-A, non-B hepatitis agent and all infectivity of the serum that contained 10(3.5) chimpanzee infective doses of hepatitis B virus were destroyed by chloroform. The chloroform-sensitive control viruses were completely inactivated, but the chloroform-resistant control viruses lost less than 0.5 log10 of infectivity. Sham-extracted non-A, non-B hepatitis agent-containing plasma was shown to maintain its infectivity in chimpanzees that had initially ...Continue Reading

References

Mar 4, 1978·Lancet·H J AlterH Popper
Apr 10, 1975·The New England Journal of Medicine·S M FeinstoneP V Holland
Jul 1, 1977·Annals of Internal Medicine·J H HoofnagleR H Purcell
Oct 1, 1975·The Journal of Infectious Diseases·L F BarkerD H Krushak
Nov 1, 1969·Journal of Virology·J L GerinR M Chanock
Nov 1, 1980·The Journal of Infectious Diseases·E Tabor, R J Gerety
Feb 1, 1981·The Journal of Infectious Diseases·J L DienstagR H Glew
Dec 1, 1981·The Journal of Infectious Diseases·S M FeinstoneR H Purcell

❮ Previous
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Citations

Jan 1, 1992·Medical Microbiology and Immunology·R ThomssenA Uy
Jan 1, 1990·Journal of Gastroenterology and Hepatology·H J Alter
Jul 1, 1994·FEMS Microbiology Reviews·R H Purcell
Aug 4, 1999·Antiviral Chemistry & Chemotherapy·R F SchinaziS Dagan
Mar 21, 2002·Immunology and Cell Biology·A J FreemanA R Lloyd
Jun 2, 2007·The Journal of Bone and Joint Surgery. British Volume·C DelloyeO Barbier
Sep 24, 2008·Blood·Harvey J Alter, Harvey G Klein
Oct 15, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Harvey J Alter
Jul 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K CristianoS M Feinstone
Jan 1, 1992·Scandinavian Journal of Gastroenterology. Supplement·G Gitnick
Jul 1, 1991·Journal of Medical Virology·D BradleyE H Cook
Jan 1, 1996·Journal of Viral Hepatitis·H YoshikuraY K Shimizu
Jan 1, 1997·Springer Seminars in Immunopathology·K M ChangF V Chisari
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jan Martin Berke, Darius Moradpour
Feb 12, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Michael Houghton
Jun 21, 2011·Annual Review of Microbiology·Catherine L Murray, Charles M Rice
Jan 1, 1990·Scandinavian Journal of Gastroenterology. Supplement·G Gitnick
Jun 21, 2003·Journal of Neurosurgery·Denis DufraneChristian Delloye
Jan 1, 1995·Journal of Viral Hepatitis·X LiE R Schiff
Feb 1, 1986·Biotechnology and Bioengineering·G Mitra, M Wong
Dec 3, 2008·American Journal of Veterinary Research·Steven Krakowka, John A Ellis
Mar 7, 2020·Clinical Liver Disease·Harvey J AlterRobert H Purcell
Jan 25, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jeremy V SuhardiEbru Oral
Nov 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R H PurcellM E Hrinda
Jan 1, 1989·Journal of Medical Virology·L F HeJ L Gerin
Jan 1, 1991·Archives of Virology·P G Plagemann
Jul 1, 1991·Journal of Gastroenterology and Hepatology·K Kiyosawa, S Furuta
Sep 17, 2013·Transfusion·Jay S EpsteinHarvey G Klein
Feb 1, 1996·Rheumatic Diseases Clinics of North America·V Agnello, P L Romain

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