PMID: 8459108Apr 2, 1993Paper

Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry

Journal of Immunological Methods
J K NicholsonJ S McDougal

Abstract

Reagents that lyse red blood cells and fix white blood cells were tested for their ability to inactivate cell-associated human immunodeficiency virus (HIV). Whole blood was spiked with cells from an HIV-positive cell line (H9), lysed, and fixed. The cell preparations were then cocultured with T cell blasts in serial ten-fold dilutions to rescue infectious virus and measure viral titer. All commercial lysing and fixing reagents tested inactivated cell-associated HIV by 3-5 logs, while ammonium chloride had little effect. Although an additional incubation with 1% formaldehyde for 30 min did not increase the effectiveness of the commercial lysing/fixing reagents, it did inactivate cell-associated HIV in blood treated with ammonium chloride.

References

Apr 17, 1990·Journal of Immunological Methods·C H Aloisio, J K Nicholson
Jan 1, 1990·Cytometry·J M CoryB M Ohlsson-Wilhelm
Dec 14, 1989·The New England Journal of Medicine·D D HoM Alam
Jan 22, 1986·Journal of Immunological Methods·J D LifsonE G Engleman
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M E HarperF Wong-Staal
Jan 1, 1981·Journal of Immunological Methods·L L Lanier, N L Warner

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Citations

Feb 10, 1994·Journal of Immunological Methods·J K Nicholson, S W Browning
Jun 21, 1996·Journal of Immunological Methods·R M BorzìA Facchini
Sep 12, 2013·Cytometry. Part B, Clinical Cytometry·Bruce H DavisUNKNOWN ICSH/ICCS Working Group
Nov 11, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ingrid SchmidStephen P Perfetto
Dec 9, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Avrill M AsplandSherry Thornton
Nov 11, 2019·Journal of Virological Methods·Fabian KrieselHanna-Mari Baldauf
Oct 1, 1994·Clinical Microbiology Reviews·J J McSharry
Jun 8, 2021·Journal of Virological Methods·Stephen McClearyHelen Crooke

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