Abstract
Supernatant obtained from granulocytes stimulated in the presence of cytochalasin B by the chemotactic peptide N-formyl-norleucyl-leucyl-phenylalanyl-norleucyl-tyrosyl- lysine displayed an inhibitory effect on the plasmin-dependent conversion of tumor urokinase-type plasminogen activator proenzyme (pro-uPA) to the active form of uPA. Moreover, the supernatant was also found to inhibit the fibrinolytic activity of human vulva (A431) and breast (MCF7) carcinoma cell lines, which contain large amounts of pro-uPA, by 87% and 96%, respectively. By using eglin C (elastase inhibitor) and a monoclonal antibody to elastase (proteolytic activity blocker of the enzyme), elastase was identified as the key enzyme of the supernatant in these phenomena. Purified elastase converted pro-uPA to an enzymatically inactive molecule composed of two polypeptide chains of Mr = 33,000 and 22,000 linked to each other by a disulfide bond. Elastase-containing granulocytes were identified by immunohistochemistry techniques in the tissues of squamous cell carcinoma and adenocarcinoma of uterus. The cells were found close to the tumor cells and in the stroma surrounding the tumor nests. By immunohistochemical staining, uPA was also found in the tumor cells. ...Continue Reading
References
Jun 1, 1975·The Journal of Experimental Medicine·R A Clark, S J Klebanoff
Jan 1, 1985·Advances in Cancer Research·K DanøL Skriver
Jan 1, 1986·Cancer Chemotherapy and Pharmacology·M FlentjeP Schlag
Feb 15, 1985·Science·S J WeissS T Test
Jan 1, 1988·Enzyme·G Markus
Sep 30, 1987·Biochemical and Biophysical Research Communications·P W Gudewicz, N Gilboa
Sep 1, 1988·Asia-Oceania Journal of Obstetrics and Gynaecology·N KanayamaK Horiuchi
Dec 31, 1987·Biochemical and Biophysical Research Communications·K Watanabe, H Nakagawa
Jul 1, 1986·The Journal of Clinical Investigation·J I WeitzF J Morgan
Nov 15, 1986·International Journal of Cancer. Journal International Du Cancer·J F CajotF Bachmann
Dec 25, 1986·The New England Journal of Medicine·H F Dvorak
Sep 1, 1986·The Journal of Experimental Medicine·J M Heiple, L Ossowski
Dec 10, 1986·Biochimica Et Biophysica Acta·H R LijnenD Collen
Aug 15, 1970·Nature·U K Laemmli
Feb 14, 1984·Biochimica Et Biophysica Acta·J KirchheimerB R Binder
Mar 1, 1984·Thrombosis Research·E Wojtecka-LukasikM Kopeć
Jul 1, 1983·The Journal of Cell Biology·N BorregaardR A Clark
Jun 1, 1984·The Journal of Clinical Investigation·V GurewichR Greenlee
Oct 15, 1982·Thrombosis Research·E K KruithofF Bachmann
Feb 1, 1982·European Journal of Cancer & Clinical Oncology·T Thorsen
Mar 1, 1981·British Journal of Haematology·I YustN Goldsher