Inactivation of lsr2 results in a hypermotile phenotype in Mycobacterium smegmatis.

Journal of Bacteriology
Kriti AroraJohn L Dahl

Abstract

Mycobacterial species are characterized by the presence of lipid-rich, hydrophobic cell envelopes. These cell envelopes contribute to properties such as roughness of colonies, aggregation of cells in liquid culture without detergent, and biofilm formation. We describe here a mutant strain of Mycobacterium smegmatis, called DL1215, which demonstrates marked deviations from the above-mentioned phenotypes. DL1215 arose spontaneously from a strain deficient for the stringent response (M. smegmatis Delta rel(Msm) strain) and is not a reversion to a wild-type phenotype. The nature of the spontaneous mutation was a single base-pair deletion in the lsr2 gene, leading to the formation of a truncated protein product. The DL1215 strain was complicated by having both inactivated rel(Msm) and lsr2 genes, and so a single lsr2 mutant was created to analyze the gene's function. The lsr2 gene was inactivated in the wild-type M. smegmatis mc(2)155 strain by allelic replacement to create strain DL2008. Strain DL2008 shows characteristics unique from those of both the wild-type and Delta rel(Msm) strains, some of which include a greatly enhanced ability to slide over agar surfaces (referred to here as "hypermotility"), greater resistance to phage ...Continue Reading

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Citations

Sep 3, 2013·Veterinary Microbiology·José A Vázquez-BolandAna Valero-Rello
May 8, 2013·Infection and Immunity·Bart A EijkelkampMelissa H Brown
Aug 3, 2010·Journal of Bacteriology·Kiet T NguyenKeith M Derbyshire
Mar 23, 2011·Journal of Bacteriology·Fanhong MengCaitilyn Allen
Jul 21, 2014·Enzyme and Microbial Technology·Navdeep GroverRavi S Kane
Jul 22, 2015·Antimicrobial Agents and Chemotherapy·Sheng-Hui TsaiShiau-Ting Hu
Oct 31, 2012·Expert Review of Anti-infective Therapy·Jun Liu, Blair R G Gordon
Apr 8, 2011·FEMS Microbiology Letters·John L Dahl, Dalia Lau Bonilla
May 23, 2019·Frontiers in Microbiology·Vincent Le MoigneJean-Louis Herrmann
Feb 5, 2021·Scientific Reports·Marta KołodziejJolanta Zakrzewska-Czerwińska
May 25, 2021·Biofouling·Esmeralda Ivonne Niño-PadillaAdriana Garibay-Escobar
May 2, 2021·Applied and Environmental Microbiology·Sara C GomesLeonilde M Moreira

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