Inactivation of Mg chelatase during transition from anaerobic to aerobic growth in Rhodobacter capsulatus.

Journal of Bacteriology
Robert D WillowsSamuel I Beale

Abstract

The facultative photosynthetic bacterium Rhodobacter capsulatus can adapt from an anaerobic photosynthetic mode of growth to aerobic heterotrophic metabolism. As this adaptation occurs, the cells must rapidly halt bacteriochlorophyll synthesis to prevent phototoxic tetrapyrroles from accumulating, while still allowing heme synthesis to continue. A likely control point is Mg chelatase, the enzyme that diverts protoporphyrin IX from heme biosynthesis toward the bacteriochlorophyll biosynthetic pathway by inserting Mg(2+) to form Mg-protoporphyrin IX. Mg chelatase is composed of three subunits that are encoded by the bchI, bchD, and bchH genes in R. capsulatus. We report that BchH is the rate-limiting component of Mg chelatase activity in cell extracts. BchH binds protoporphyrin IX, and BchH that has been expressed and purified from Escherichia coli is red in color due to the bound protoporphyrin IX. Recombinant BchH is rapidly inactivated by light in the presence of O(2), and the inactivation results in the formation of a covalent adduct between the protein and the bound protoporphyrin IX. When photosynthetically growing R. capsulatus cells are transferred to aerobic conditions, Mg chelatase is rapidly inactivated, and BchH is th...Continue Reading

References

Nov 7, 1975·Archives of Microbiology·P F WeaverH Gest
Jun 1, 1992·Photochemistry and Photobiology·J Moan, K Berg
Aug 13, 1968·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A GorcheinG H Tait
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S I BealeV Dzelzkalns
Apr 15, 1994·Journal of Molecular Biology·D W BollivarC E Bauer
May 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·K KönigR Steiner
Mar 7, 1996·Molecular & General Genetics : MGG·P E JensenK W Henningsen
Jul 27, 2001·Journal of Photochemistry and Photobiology. B, Biology·L MaJ Moan
Feb 1, 1957·Journal of Cellular Physiology·G COHEN-BAZIRER Y STANIER

❮ Previous
Next ❯

Citations

Jul 2, 2009·Photosynthesis Research·Aline Gomez Maqueo ChewDonald A Bryant
Oct 13, 2016·Journal of the American Society for Mass Spectrometry·Cosima Damiana CalvanoFrancesco Palmisano
Mar 21, 2007·Photosynthesis Research·David W Bollivar
May 5, 2007·Prikladnaia biokhimiia i mikrobiologiia·E V PatrushevaA A Tsygankov
Feb 12, 2008·The Journal of Biological Chemistry·Nickolche SirijovskiMats Hansson
Mar 31, 2004·The Journal of Biological Chemistry·James D Reid, C Neil Hunter
Sep 16, 2008·The Journal of Biological Chemistry·Artur Sawicki, Robert D Willows

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.