Inactivation of Plasmodium falciparum in whole blood using the amustaline and glutathione pathogen reduction technology

Transfusion
Cissé SowPhilippe Grellier

Abstract

Risk of transfusion-transmitted (TT) malaria is mainly associated with whole blood (WB) or red blood cell (RBC) transfusion. Risk mitigation relies mostly on donor deferral while a limited number of countries perform blood testing, both negatively impacting blood availability. This study investigated the efficacy of the pathogen reduction system using amustaline and glutathione (GSH) to inactivate Plasmodium falciparum in WB. WB units were spiked with ring stage P. falciparum infected RBCs. Parasite loads were measured in samples at time of infection, after 24 hours at room temperature (RT), and after a 24-hour incubation at RT post-treatment with 0.2 mM amustaline and 2 mM GSH. Serial 10-fold dilutions of the samples were inoculated to RBC cultures and maintained up to 4 weeks. Parasitemia was quantified by cytometry. The P. falciparum viability assay has a limit of detection of a single live parasite per sample. Input parasite titer was >5.7 log10 TCID50 per mL. A 24-hour incubation at RT paused parasite development in controls, but they retained viability and infectivity when tested in culture. In contrast, no infectious parasites were detected in the amustaline/GSH-treated sample after 4 weeks of culture. A robust level of ...Continue Reading

References

Jan 1, 1989·The Journal of Experimental Medicine·D Kwiatkowski
Jul 27, 2002·Emerging Infectious Diseases·T HänscheidM P Grobusch
Jan 25, 2006·Vox Sanguinis·A D Kitchen, P L Chiodini
May 27, 2009·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Andrea K BoggildW Conrad Liles
Dec 10, 2009·Biologicals : Journal of the International Association of Biological Standardization·N A MuftiL Lin
Dec 17, 2009·Nature Protocols·Azar RadfarJosé M Bautista
Jul 23, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Reinhard HenschlerNina Mufti
Sep 20, 2012·Methods in Molecular Biology·Anthony BouillonJean-Christophe Barale
May 10, 2013·Transfusion·Mira El ChaarJean-Pierre Allain
Apr 5, 2016·Hematology/oncology Clinics of North America·David J RobertsImelda Bates
Apr 9, 2016·The Journal of Community and Supportive Oncology·Jeremy M O'ConnorJonas A de Souza
Sep 6, 2017·Transfusion·Laura TonnettiSusan L Stramer
Jan 18, 2018·Malaria Journal·Federica VerraZeno Bisoffi
Jul 25, 2019·Open Forum Infectious Diseases·Ehsan AhmadpourMuge Cevik

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
Assay

Software Mentioned

INTERCEPT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.