Inactivation of potato lipoxygenase by hydroperoxy acids as suicide substrates

Biochemical and Biophysical Research Communications
M R KimD E Sok

Abstract

The preincubation of potato lipoxygenase with 9(S)-hydroperoxyoctadecatrienoic acid, 15(S)-hydroperoxyeicosatetraenoic acid or 5(S)-hydroperoxyeicosatetraenoic acid which can be subjected to further lipoxygenation led to the gradual inactivation of the lipoxygenase activity, whereas 13(S)-hydroperoxy-9,13,15-octadecatrienoic acid or 15(S)-hydroperoxy-11,13,17-eicosatrienoic acid had no significant effect. The inhibitory effect of the peroxy acids was abolished by hemoglobin. Based on these observations, it is proposed that the unstable epoxide intermediates from the respective peroxy acids may be responsible for the inactivation of potato lipoxygenase. In the comparative study, it was found that 15(S)-hydroperoxyeicosatetraenoic acid possessed more effective inhibitory role than the other acids with Ki value of 250 microM.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Borgeat, B Samuelsson
Aug 15, 1989·Biochemical and Biophysical Research Communications·D E Sok, M R Kim
Sep 16, 1985·Biochemical and Biophysical Research Communications·D J NathanielA W Ford-Hutchinson
Jul 19, 1973·Biochimica Et Biophysica Acta·M Roza, A Francke
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T ShimizuB Samuelsson

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Citations

Jul 30, 1991·Biochimica Et Biophysica Acta·H W Gardner
Sep 20, 2005·Biochemical and Biophysical Research Communications·Hartmut KuhnMatthias Walther
Aug 6, 2008·Journal of Agricultural and Food Chemistry·Long Shuang HuangDai-Eun Sok

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