Inactivation of respiratory syncytial virus by zinc finger reactive compounds.

Virology Journal
Marina S BoukhvalovaJorge C G Blanco

Abstract

Infectivity of retroviruses such as HIV-1 and MuLV can be abrogated by compounds targeting zinc finger motif in viral nucleocapsid protein (NC), involved in controlling the processivity of reverse transcription and virus infectivity. Although a member of a different viral family (Pneumoviridae), respiratory syncytial virus (RSV) contains a zinc finger protein M2-1 also involved in control of viral polymerase processivity. Given the functional similarity between the two proteins, it was possible that zinc finger-reactive compounds inactivating retroviruses would have a similar effect against RSV by targeting RSV M2-1 protein. Moreover, inactivation of RSV through modification of an internal protein could yield a safer whole virus vaccine than that produced by RSV inactivation with formalin which modifies surface proteins. Three compounds were evaluated for their ability to reduce RSV infectivity: 2,2'-dithiodipyridine (AT-2), tetraethylthiuram disulfide and tetramethylthiuram disulfide. All three were capable of inactivating RSV, with AT-2 being the most potent. The mechanism of action of AT-2 was analyzed and it was found that AT-2 treatment indeed results in the modification of RSV M2-1. Altered intramolecular disulfide bond f...Continue Reading

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Citations

Feb 4, 2011·The Journal of General Virology·Tomas StrandinHilkka Lankinen
Oct 10, 2015·Computational and Mathematical Methods in Medicine·Gilberto González-Parra, Hana M Dobrovolny
Aug 12, 2015·Antiviral Research·Michela CancellieriAndrea Brancale
Feb 16, 2016·Journal of Inorganic Biochemistry·Patricia B Lutz, Craig A Bayse
Mar 23, 2017·Topics in Current Chemistry·Brian C Shook, Kai Lin

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Methods Mentioned

BETA
nuclear magnetic resonance
electrophoresis

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