Inactivation of sll1556 in Synechocystis strain PCC 6803 impairs isoprenoid biosynthesis from pentose phosphate cycle substrates in vitro

Journal of Bacteriology
Kelly PoliquinE Gantt

Abstract

In cyanobacteria many compounds, including chlorophylls, carotenoids, and hopanoids, are synthesized from the isoprenoid precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate. Isoprenoid biosynthesis in extracts of the cyanobacterium Synechocystis strain PCC 6803 grown under photosynthetic conditions, stimulated by pentose phosphate cycle substrates, does not appear to require methylerythritol phosphate pathway intermediates. The sll1556 gene, distantly related to type 2 IPP isomerase genes, was disrupted by insertion of a Kanr cassette. The mutant was fully viable under photosynthetic conditions although impaired in the utilization of pentose phosphate cycle substrates. Compared to the parental strain the Deltasll1556 mutant (i) is deficient in isoprenoid biosynthesis in vitro with substrates including glyceraldehyde-3-phosphate, fructose-6-phosphate, and glucose-6-phosphate; (ii) has smaller cells (diameter ca. 13% less); (iii) has fewer thylakoids (ca. 30% less); and (iv) has a more extensive fibrous outer wall layer. Isoprenoid biosynthesis is restored with pentose phosphate cycle substrates plus the recombinant Sll1556 protein in the Deltasll1556 supernatant fraction. IPP isomerase activity could not be de...Continue Reading

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Citations

Nov 2, 2013·Chemical Reviews·Alexander R MoiseEleanore T Wurtzel
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Félix J SangariManuel Rodríguez-Concepción
Nov 18, 2004·Journal of Bacteriology·Sam J BarkleyC Dale Poulter
Mar 15, 2014·PloS One·Elias EnglundPia Lindberg
Nov 20, 2012·Annual Review of Plant Biology·Elisabeth Gantt
Mar 27, 2012·Journal of Biotechnology·Iara M P Machado, Shota Atsumi
May 11, 2005·Biochemical and Biophysical Research Communications·Masood Ahmed SiddiquiShinsuke Fujiwara
Apr 21, 2016·Biotechnology for Biofuels·Nadin PadeMartin Hagemann
Feb 11, 2014·Photosynthesis Research·Fiona K DaviesMatthew C Posewitz
Jan 24, 2015·Life·Bagmi Pattanaik, Pia Lindberg
Apr 26, 2013·The Biochemical Journal·Jordi Pérez-Gil, Manuel Rodríguez-Concepción
Sep 29, 2004·The Journal of Biological Chemistry·María B CasseraAlejandro M Katzin
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara

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