Inactivation of the global regulator LaeA in Monascus ruber results in a species-dependent response in sporulation and secondary metabolism

Fungal Biology
Qingpei LiuFusheng Chen

Abstract

The nuclear regulator LaeA has been proven to globally govern fungal development and secondary metabolism, but its function may be species-dependent, even though its amino acid sequences are well conserved in numerous fungi. Herein we identified the LaeA in Monascus ruber M7 (MrLaeA), and verified its role to mediate growth, sporulation and secondary metabolism. Results showed that the radial growth rate of the selected MrlaeA knock-out mutant (MrΔlaeA-22) was significantly faster than that of the parental strain M. ruber M7, and growth was accompanied by the formation of an abnormal colony phenotype with more abundant aerial hyphae. Interestingly, conidia production of the MrΔlaeA-22 strain was about thrice that of M. ruber M7, but ascospores were not observed in the MrΔlaeA-22 strain. Additionally, compared to M. ruber M7, MrΔlaeA-22 exhibited drastically reduced production of multiple secondary metabolites, especially those of the six well-known Monascus pigments and citrinin. Simultaneously, the selected MrlaeA complementation strain (MrΔlaeA::laeA-45) nearly recovered the capacity for sporulation and secondary metabolism observed in the parental strain. These results demonstrate that MrLaeA regulates not only secondary met...Continue Reading

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Citations

Jul 1, 2016·World Journal of Microbiology & Biotechnology·Gong Chen, Zhenqiang Wu
Nov 8, 2016·Fungal Genetics and Biology : FG & B·Pranav Chettri, Rosie E Bradshaw
Mar 2, 2018·The Journal of Microbiology·Qingqing YanYanchun Shao
Mar 9, 2018·Molecular Plant Pathology·Christelle El Hajj AssafOlivier Puel
May 3, 2019·Applied Microbiology and Biotechnology·Chan ZhangChengtao Wang
Dec 22, 2016·Journal of Agricultural and Food Chemistry·Liling WangFusheng Chen
Feb 9, 2019·Journal of Agricultural and Food Chemistry·Chan ZhangChengtao Wang

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