PMID: 9188551Jul 1, 1997Paper

Inactivation of the human immunodeficiency virus type 1 inhibitory elements allows Rev-independent expression of Gag and Gag/protease and particle formation

Journal of Virology
R SchneiderG N Pavlakis

Abstract

The expression of gag, pol, and env of human immunodeficiency virus type 1 (HIV-1) depends on the presence of the viral Rev protein. This dependence is, at least in part, due to the presence of negatively acting sequences (inhibitory or instability elements [INS]) located within unspliced and partially spliced mRNAs. The positive interaction of Rev with the Rev-responsive element in these mRNAs counteracts the negative effects of the inhibitory sequences. Here, we demonstrate that in addition to the previously identified INS1 within p17gag, several other INS elements exist within the gag/pol region of HIV-1. These elements act independently of each other and were eliminated by mutagenesis after the introduction of multiple point mutations not affecting the coding region, leading to constitutive high levels of Gag expression. Expression vectors containing an intact or nearly intact p55gag region allowed the production of immature viral particles in mammalian cells in the absence of any other HIV proteins. The introduction of additional mutations in the protease region allowed efficient production of Gag/protease, which resulted in processing of the Pr55gag precursor and production of mature Gag particles with a lentivirus-like c...Continue Reading

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Citations

Nov 16, 2001·AIDS Research and Human Retroviruses·F J IbarrondoP Krogstad
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Z HelG Franchini
Jan 23, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Yaoxing HuangDavid D Ho
Aug 13, 2008·Expert Opinion on Biological Therapy·L Jean Patterson, Marjorie Robert-Guroff
Apr 15, 2008·Experimental Biology and Medicine·Harlan RobinsArnold J Levine
Apr 30, 2010·Journal of Virology·Maik BlissenbachKlaus Uberla
Mar 19, 2013·PLoS Pathogens·Sebla B KutluayPaul D Bieniasz
Oct 24, 2014·Journal of Virology·Sophie SagnierLucile Espert
Jan 1, 2012·Biology·Anna Kula, Alessandro Marcello
Oct 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Young C ShinRonald C Desrosiers
Nov 26, 2016·Viruses·Daniela Toro-AscuyRicardo Soto-Rifo
Feb 13, 2002·AIDS Patient Care and STDs·Mario R Mautino, Richard A Morgan
Aug 6, 2004·The Journal of Biological Chemistry·Véronique BériaultAndrew J Mouland
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arguinaldo R PintoHildegund C J Ertl
Mar 30, 2007·Journal of Virology·Samuel J RulliAlan Rein
Aug 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cristina BergamaschiGeorge N Pavlakis
Jul 21, 2011·Retrovirology·Venkat S R K Yedavalli, Kuan-Teh Jeang
Dec 24, 2011·PLoS Pathogens·Martin LehmannVincent Piguet

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