Inactivation of the replication-termination system affects the replication mode and causes unstable maintenance of plasmid R1

Molecular Microbiology
M KrabbeKurt Nordström

Abstract

Two so-called Ter sites, which bind the Escherichia coli Tus protein, are located near the replication origin of plasmid R1. Inactivation of the tus gene caused a large decrease in the stability of maintenance of the R1 mini-derivative pOU47 despite the presence of a functional partition system on the plasmid. Deletion of the right Ter site caused a drop in stability similar to that observed after inactivation of the tus gene. Substitution of 2bp required for Tus binding also caused unstable plasmid maintenance, whereas no effects on stability were observed when the left Ter site was deleted. Inactivation of the tus gene was coupled to an increased occurrence of multimeric plasmid forms as shown by gel electrophoresis of pOU47 DNA. Inactivation of the recA gene did not increase plasmid stability, suggesting that the multimerization was not mediated by RecA. Plasmid DNA was isolated from the tus strain carrying plasmid pOU47 and from a wild-type strain carrying pOU47 in which the right Ter site had been inactivated; in both cases, electron microscopy revealed the presence of multimers as well as rolling-circle structures with double-stranded tails. Thus, the right Ter site in plasmid R1 appears to stabilize the plasmid by preven...Continue Reading

Citations

Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·K P LemonA D Grossman
Jan 26, 2002·Nucleic Acids Research·L OlavarrietaJ B Schvartzman
May 25, 2002·Journal of Bacteriology·Takahiro MurataTetsuya Hayashi
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Ekaterina V Mirkin, Sergei M Mirkin
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Cameron NeylonNicholas E Dixon
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·S MuluguD Bastia
Jun 23, 2009·Molecular Microbiology·Christian J RudolphRobert G Lloyd
Nov 21, 2008·Molecular Microbiology·Iain G DugginThomas M Hill
Jan 23, 2010·DNA Repair·Christian J RudolphRobert G Lloyd
Apr 4, 2015·Nucleus·Rachael BaileyAgnieszka Gambus
Oct 4, 2005·Plasmid·Kurt Nordström
May 9, 2014·Seminars in Cell & Developmental Biology·Deepak Bastia, Shamsu Zaman
Mar 4, 1998·Molecular Microbiology·J N Volff, J Altenbuchner
Dec 14, 2018·Nucleic Acids Research·Sarah L Midgley-SmithChristian J Rudolph
Apr 21, 1999·Molecular Microbiology·D E Bussiere, D Bastia
May 1, 2020·Frontiers in Microbiology·Aisha H SyedaChristian J Rudolph
Jun 25, 2015·Microbiology Spectrum·Sabine Brantl
Aug 8, 2021·International Journal of Molecular Sciences·Daniel J GoodallChristian J Rudolph

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.