Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence

Molecular Microbiology
Hélène BierneEuropean Listeria Genome Consortium

Abstract

During infection of their hosts, Gram-positive bacteria express surface proteins that serve multiple biological functions. Surface proteins harbouring a C-terminal sorting signal with an LPXTG motif are covalently linked to the cell wall peptidoglycan by a transamidase named sortase. Two genes encoding putative sortases, termed srtA and srtB, were identified in the genome of the intracellular pathogenic bacterium Listeria monocytogenes. Inactivation of srtA abolishes anchoring of the invasion protein InlA to the bacterial surface. It also prevents the proper sorting of several other peptidoglycan-associated LPXTG proteins. Three were identified by a mass spectrometry approach. The DeltasrtA mutant strain is defective in entering epithelial cells, similar to a DeltainlA mutant. In contrast to a DeltainlA mutant, the DeltasrtA mutant is impaired for colonization of the liver and spleen after oral inoculation in mice. Thus, L. monocytogenes srtA is required for the cell wall anchoring of InlA and, presumably, for the anchoring of other LPXTG-containing proteins that are involved in listerial infections.

References

Jan 1, 1991·Proteins·G D SchulerD J Lipman
Jan 1, 1994·Methods in Enzymology·J L GaillardP Cossart
Apr 1, 1993·Molecular Microbiology·A CamilliD A Portnoy
Nov 1, 1996·Science·K IretonP Cossart
Dec 16, 1998·Trends in Biochemical Sciences·S F Altschul, E V Koonin
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·H Ton-ThatO Schneewind
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·P Cossart, R Jonquières
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·S K MazmanianO Schneewind
Jan 13, 2001·Current Opinion in Immunology·P Cossart, H Bierne
Mar 10, 2001·Trends in Microbiology·M J PallenK Dunbar
Jun 13, 2001·Molecular Microbiology·S K MazmanianO Schneewind
Oct 27, 2001·Science·P GlaserP Cossart

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Citations

Aug 22, 2008·Archives of Microbiology·Changjun WangJiaqi Tang
Nov 29, 2011·Applied Microbiology and Biotechnology·Yuhua ChangLynne McLandsborough
Apr 6, 2004·Comptes rendus biologies·Javier Pizarro-CerdáPascale Cossart
Jan 24, 2004·Microbes and Infection·Hartmut H NiemannDirk W Heinz
Mar 24, 2004·Trends in Microbiology·Gavin K Paterson, Timothy J Mitchell
Jan 17, 2004·Lancet·Philippe Moreillon, Yok-Ai Que
Apr 26, 2002·Trends in Microbiology·Didier CabanesPascale Cossart
Oct 19, 2013·International Journal of Oral Science·Ming-Yun LiRichard L Gregory
May 20, 2006·Nature Reviews. Microbiology·Mélanie HamonPascale Cossart
Oct 2, 2002·Molecular Microbiology·Philippe GlaserFrank Kunst
Aug 19, 2011·The Journal of Biological Chemistry·Francisco García-del PortilloM Graciela Pucciarelli
Apr 1, 2008·The Journal of Biological Chemistry·Matthew L BentleyDewey G McCafferty
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Olaf Schneewind, Dominique M Missiakas
Jun 6, 2006·Applied and Environmental Microbiology·Jan-Peter van PijkerenPaul W O'Toole
Mar 24, 2010·Infection and Immunity·Divya IyerJanina P Lewis
Feb 24, 2010·Infection and Immunity·Nicolas PersonnicHélène Bierne
Apr 23, 2004·Infection and Immunity·David Comfort, Robert T Clubb
Sep 24, 2005·Infection and Immunity·Christophe SabetHélène Bierne
Jul 26, 2005·Infection and Immunity·Steven D Zink, Drusilla L Burns
Oct 17, 2006·Journal of Bacteriology·Victor J TorresEric P Skaar
Mar 19, 2004·Journal of Bacteriology·Hélène BierneAlain Charbit
Jul 5, 2005·Journal of Bacteriology·Jos BoekhorstRoland J Siezen
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Hélène Bierne, Pascale Cossart
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Luciano A MarraffiniOlaf Schneewind
Jun 7, 2006·Annual Review of Microbiology·June R Scott, Timothy C Barnett
Nov 15, 2011·PloS One·Willy AucherAgnès Fouet
Aug 29, 2013·Current Microbiology·Ping HuangWei Min Chen
Sep 5, 2014·Microbial Cell Factories·Marie-Pierre Chapot-Chartier, Saulius Kulakauskas
Jan 31, 2016·Biochemical Pharmacology·Hongen LiXuming Deng
Aug 19, 2015·Journal of Medicinal Chemistry·Stella CascioferroGiuseppe Daidone
Dec 28, 2007·International Journal of Food Microbiology·Andrea GianottiMaria Elisabetta Guerzoni

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