PMID: 3052299Oct 1, 1988

Inactivation of the thiamine transport system in Saccharomyces cerevisiae with O-bromoacetylthiamine

Archives of Biochemistry and Biophysics
H NishimuraA Iwashima


We have synthesized and characterized O-bromoacetylthiamine (BrAcThiamine), a new reagent for inactivating the thiamine transport system in Saccharomyces cerevisiae. A Lineweaver-Burk plot of data from the transport kinetic measurements showed that BrAcThiamine was a competitive inhibitor of thiamine transport in S. cerevisiae with a Ki value of 0.60 microM. Incubating BrAcThiamine with yeast cells at 40 degrees C in 0.05 M potassium phosphate buffer, pH 5.0, caused concentration- and time-dependently a remarkable loss of thiamine transport activity. The inactivating reaction of yeast thiamine transport by BrAcThiamine proceeded most effectively at pH 5.0, coinciding with the optimal pH of the transport activity. Thiamine and thiamine analogs (pyrithiamine and O-acetylthiamine) protected yeast thiamine transport activity against the inactivation by BrAcThiamine. In addition, it was found that a membrane fraction prepared from yeast cells treated with BrAcThiamine had a thiamine-binding activity only 20% of that from control cells without inactivating the binding activity of the soluble fraction. These results suggest that BrAcThiamine inactivates the uptake activity by irreversible binding to the binding site of carrier protein...Continue Reading


Mar 27, 1979·Biochimica Et Biophysica Acta·A Iwashima, H Nishimura
Nov 2, 1979·Biochimica Et Biophysica Acta·A IwashimaY Nose
Jul 29, 1987·Biochimica Et Biophysica Acta·S ShigeokaS Kitaoka
Jun 15, 1986·Experientia·H NishimuraA Iwashima
Dec 13, 1973·Biochimica Et Biophysica Acta·A IwashimaY Nose
Nov 23, 1983·Biochimica Et Biophysica Acta·E BellionB R McKellar

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Hydrogen-Ion Concentration
Saccharomyces cerevisiae
Thiamine Mononitrate

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