Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.)

The Plant Journal : for Cell and Molecular Biology
Mi-Ok WooHee-Jong Koh

Abstract

A rice genic male-sterility gene ms-h is recessive and has a pleiotropic effect on the chalky endosperm. After fine mapping, nucleotide sequencing analysis of the ms-h gene revealed a single nucleotide substitution at the 3'-splice junction of the 14th intron of the UDP-glucose pyrophosphorylase 1 (UGPase1; EC2.7.7.9) gene, which causes the expression of two mature transcripts with abnormal sizes caused by the aberrant splicing. An in vitro functional assay showed that both proteins encoded by the two abnormal transcripts have no UGPase activity. The suppression of UGPase by the introduction of a UGPase1-RNAi construct in wild-type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms-h mutant phenotype, whereas overexpression of UGPase1 in ms-h mutant plants restored male fertility and the transformants produced T(1) seeds that segregated into normal and chalky endosperms. In addition, both phenotypes were co-segregated with the UGPase1 transgene in segregating T(1) plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm. Our results suggest that UGPase1 plays a key role in pollen development as well a...Continue Reading

References

Feb 10, 1995·The Journal of Biological Chemistry·S M Berget
Aug 1, 1993·Molecular and Cellular Biology·H LouM A Schuler
Jun 24, 1993·Nature·M G AartsA Pereira
Mar 1, 1993·Molecular and Cellular Biology·A J McCulloughM A Schuler
Feb 6, 1998·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·K J RossG H Jones
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·S D Michaels, R M Amasino
Sep 23, 1998·The Plant Journal : for Cell and Molecular Biology·M IsshikiK Shimamoto
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·J GloverE Dennis
Aug 11, 2000·Plant Science : an International Journal of Experimental Plant Biology·J WangJ H Oard
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·M GoetzT Roitsch
Feb 13, 2002·Current Biology : CB·David Baulcombe
Jan 1, 2003·Journal of Experimental Botany·T RoitschA K Sinha
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T. AbeT. Sasahara
Jun 27, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y G WangB Wang
Mar 17, 2004·Plant Physiology·Leszek A KleczkowskiHenrik Johansson
Jan 22, 2005·Plant & Cell Physiology·Nori KurataYukihiro Ito
Jun 29, 2005·Journal of Molecular Evolution·Xuemei JiJohn Bennett
Apr 15, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junfang ChenC C Jan
Dec 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S R McCouchS D Tanksley

❮ Previous
Next ❯

Citations

Aug 20, 2009·Transgenic Research·Heather D ColemanShawn D Mansfield
Dec 21, 2012·Glycobiology·Jana FühringRita Gerardy-Schahn
Apr 16, 2009·Plant & Cell Physiology·Meng MengLeszek A Kleczkowski
Jul 10, 2009·Yi chuan = Hereditas·Li-Jun ZhouJian-Min Wan
Mar 11, 2011·Trends in Biotechnology·Charles KwitC Neal Stewart
Feb 11, 2015·Journal of Experimental Botany·Nese SreenivasuluPolavarpu B Kavi Kishor
Jul 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Tatsuro HiroseRyu Ohsugi
Jun 19, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Quanlin LiZheng Yuan
Dec 1, 2015·Scientific Reports·Shu-Ye JiangSrinivasan Ramachandran
May 31, 2019·Journal of Experimental Botany·Gopal MisraNese Sreenivasulu
Mar 28, 2020·The Plant Journal : for Cell and Molecular Biology·Rehenuma TabassumTomoyuki Katsube-Tanaka
Mar 17, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xianjin QiuZhikang Li
Aug 10, 2017·Molecular Biotechnology·Pawan ShuklaPulugurtha Bharadwaja Kirti
Jun 29, 2018·International Journal of Genomics·Yong-Mei JinXiu-Feng Lin

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF249880

Methods Mentioned

BETA
PCR
transgenic
electrophoresis
glycosylation
enzymatic assay

Software Mentioned

map maker

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.