Incidence of apoptosis and transcript abundance in bovine follicular cells is associated with the quality of the enclosed oocyte

Theriogenology
D JanowskiM Hölker

Abstract

The close contact and interaction between the oocyte and the follicular environment influence the establishment of oocyte developmental competence. Moreover, it is assumed that apoptosis in the follicular cells has a beneficial influence on the developmental competence of oocytes. The aim of this study was to investigate whether bovine oocytes with varied developmental competence show differences in the degree of apoptosis and gene expression pattern in their surrounding follicular cells (cumulus and granulosa cells). Oocytes and follicular cells from follicles of 3 to 5 mm in diameter were grouped as brilliant cresyl blue (BCB)+ and BCB- based on glucose-6-phosphate dehydrogenase (G6PDH) activity in the ooplasm by BCB staining. In the follicular cells initial, early and late apoptotic events were assessed by analyzing caspase-3 activity, annexin-V and TUNEL, respectively. Global gene expression was investigated in immature oocytes and corresponding follicular cells. BCB+ oocytes resulted in a higher blastocyst rate (19.3%) compared to the BCB- group (7.4%, P < 0.05). Moreover, the analysis of apoptosis showed a higher caspase-3 activity in the follicular cells and an increased degree of late apoptotic events in granulosa cells...Continue Reading

References

Dec 1, 1990·Molecular Reproduction and Development·J Motlík, M Kubelka
Mar 1, 1986·Journal of Reproduction and Fertility·P HyttelT Greve
Aug 1, 1995·Molecular Reproduction and Development·P SutovskýA Pavlok
May 1, 1995·Molecular Reproduction and Development·P Blondin, M A Sirard
Dec 1, 1994·Endocrine Reviews·A J HsuehA Tsafriri
Dec 1, 1995·Molecular Reproduction and Development·T FairT Greve
Aug 15, 1997·The Biochemical Journal·G M Cohen
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Sep 30, 1998·Cell·D R Green
Oct 31, 1998·Reproduction, Nutrition, Development·M A Driancourt, B Thuel
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Mar 29, 2000·Theriogenology·P J HendriksenS J Dieleman
Mar 31, 2000·Experimental Cell Research·S Nagata
Feb 13, 2001·Trends in Biochemical Sciences·J M Adams, S Cory
Sep 26, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·C VozziJ A Haefliger
Nov 3, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·H F Irving-RodgersR J Rodgers
Jun 6, 2002·Animal Reproduction Science·Sylvie Bilodeau-Goeseels, Paul Panich
Nov 6, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·M A Fenwick, P R Hurst
Jun 4, 2004·Nature Biotechnology·Zhijin Wu, Rafael A Irizarry
Oct 30, 2004·The Journal of Reproduction and Development·Noboru ManabeTakashi Miyano
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·E EliyahuR Shalgi
Apr 12, 2005·Reproduction in Domestic Animals = Zuchthygiene·Jb FeranilT Nakao
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Salilew-Wondim DessieDawit Tesfaye
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Helmut TornerDawit Tesfaye
Feb 6, 2010·Reproductive Biomedicine Online·M FilaliV Machelon

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Citations

Dec 21, 2013·Journal of Assisted Reproduction and Genetics·Amir Hossein Abazari-KiaMohammad Salehi
Sep 10, 2013·Reproductive Biology·Jolanta Opiela, Lucyna Kątska-Książkiewicz
Jun 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·T H C De BemC L V Leal
Jul 31, 2013·British Journal of Pharmacology·Shuntaro Ikeda, Masayasu Yamada
Dec 21, 2013·Zygote : the Biology of Gametes and Early Embryos·E C S SantosH Iwata
Jun 18, 2015·PloS One·Huihua WangLixin Du
Apr 15, 2016·Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine·Armin AhmedArvind Kumar Baronia
Jan 28, 2020·Molecular Reproduction and Development·Maria F TelloDaniel M Lombardo
Sep 29, 2020·Zygote : the Biology of Gametes and Early Embryos·Seda KarabulutIlknur Keskin
Jun 22, 2017·Frontiers in Physiology·Yanzhenzi DaiRavinder Anand-Ivell
Jun 28, 2019·Reproduction, Fertility, and Development·M S LorenzoD M Lombardo

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