Abstract
To determine the incidence of the mixed lineage leukemia (MLL) gene rearrangements in acute myeloid leukemia (AML) without cytogenetically-detected 11q23 abnormalities, we screened 64 cases of AML at diagnosis for MLL rearrangement by FISH. Three cases (4.7%) had a MLL rearrangement detected; one was shown to have a cryptic t(11;22)(q23;q11) and another to have a t(9;11)(p21-22;q23) which had been missed by the conventional cytogenetic study. No 11q23 structural abnormality was visible in the third case. Twenty-six of the 64 cases were further studied by Southern blotting and DNA hybridization, and four of these cases (15%) were found to have MLL rearrangement: in three of these, FISH had not detected any abnormality. FISH was also used to confirm MLL involvement in eight cases of AML that had a cytogenetic abnormality at 11q23; in one of these, Southern blot did not show a rearrangement. The survival of patients with MLL abnormalities identified by cytogenetics, FISH and/or DNA analysis was significantly worse than that of patients without MLL abnormalities (event-free survival p = 0.016) although two patients with a t(9;11)(p21-22;q23) were long-term survivors, consistent with this particular translocation having a better pro...Continue Reading
References
Jan 1, 1977·British Journal of Cancer·R PetoP G Smith
Sep 1, 1992·Leukemia Research
Oct 1, 1994·Genes, Chromosomes & Cancer·S E HeightD Catovsky
Jun 1, 1995·Genes, Chromosomes & Cancer·O A Bernard, R Berger
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·V SahaB D Young
Aug 1, 1993·Genes, Chromosomes & Cancer·H KobayashiJ D Rowley
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·M H DreylingS K Bohlander
Jun 1, 1996·British Journal of Haematology·N J PhilpottD Catovsky
Sep 18, 1997·Genes, Chromosomes & Cancer·J R MüllerP F Hausner
Mar 3, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M A ChaudM F Gimeno
May 21, 1998·Leukemia·G J SwansburyL M Secker-Walker
May 21, 1998·Leukemia·D M LillingtonL M Secker-Walker
May 21, 1998·Leukemia·C J HarrisonL M Secker-Walker
Jun 20, 1998·British Journal of Haematology·K HeinonenC D Bloomfield
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·B D YuS J Korsmeyer
Sep 16, 1998·Leukemia·C C SillimanS P Hunger
Feb 3, 1999·Annual Review of Genetics·J D Rowley
Apr 30, 1999·Genes, Chromosomes & Cancer·K KumonY Kaneko
Nov 11, 1999·British Journal of Haematology·M NaritaM Ohki
Dec 22, 1999·Leukemia·M van der BurgJ J van Dongen
Jan 19, 2000·Leukemia·S K BohlanderM H Dreyling
Jan 19, 2000·Leukemia·K M CarlsonJ D Rowley
Citations
Jun 18, 2003·Cancer Genetics and Cytogenetics·Mirjam KlausClaudia Schoch
Dec 21, 2007·Leukemia·D Caudell, P D Aplan
Jan 22, 2005·The New England Journal of Medicine·Karen K Ballen, Robert P Hasserjian
Jul 5, 2005·Cancer Genetics and Cytogenetics·Valérie BeyerMartine Jotterand
Feb 24, 2006·Oncogene·L Fröhlich ArchangeloS K Bohlander
Dec 22, 2015·Leukemia·S DuttaS K Bohlander
Jun 17, 2014·Oncology Letters·Wen GaoWen Ming Chen