Incidence of respiratory viruses in Peruvian children with acute respiratory infections.

Journal of Medical Virology
Juana del Valle MendozaTomás Pumarola

Abstract

Acute respiratory infections are responsible for high morbi-mortality in Peruvian children. However, the etiological agents are poorly identified. This study, conducted during the pandemic outbreak of H1N1 influenza in 2009, aims to determine the main etiological agents responsible for acute respiratory infections in children from Lima, Peru. Nasopharyngeal swabs collected from 717 children with acute respiratory infections between January 2009 and December 2010 were analyzed by multiplex RT-PCR for 13 respiratory viruses: influenza A, B, and C virus; parainfluenza virus (PIV) 1, 2, 3, and 4; and human respiratory syncytial virus (RSV) A and B, among others. Samples were also tested with direct fluorescent-antibodies (DFA) for six respiratory viruses. RT-PCR and DFA detected respiratory viruses in 240 (33.5%) and 85 (11.9%) cases, respectively. The most common etiological agents were RSV-A (15.3%), followed by influenza A (4.6%), PIV-1 (3.6%), and PIV-2 (1.8%). The viruses identified by DFA corresponded to RSV (5.9%) and influenza A (1.8%). Therefore, respiratory syncytial viruses (RSV) were found to be the most common etiology of acute respiratory infections. The authors suggest that active surveillance be conducted to identif...Continue Reading

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Citations

Jun 3, 2015·Nutrients·Claire L Greiller, Adrian R Martineau
Jul 5, 2016·Reviews in Medical Virology·Alireza TahamtanVahid Salimi
Dec 27, 2016·International Journal of Pediatrics·Pravakar MishraAmitabh Singh
May 24, 2019·BioMed Research International·Serges TchatchouangRichard Njouom

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Methods Mentioned

BETA
nucleic acid amplification
PCR
reverse transcription PCR
Assay

Software Mentioned

SPSS Statistics

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