PMID: 7011568Jan 1, 1981Paper

Incompatibility properties of Col E1 and pMB1 derivative plasmids: random replication of multicopy replicons

Cell
T Hashimoto-Gotoh, K N Timmis

Abstract

The incompatibility properties of Col E1-like plasmids have been examined in Rec+ and RecA- bacteria. Two Col E1- (or two pMB1-) derivative plasmids coreplicated in the same clone for many cell doublings, irrespective of the rec genotype of host bacteria. Their kinetics of segregation were found to be consistent with models that assume a random choice of template molecule for each plasmid replication event, but with models based on a single (master) template molecule per cell. In contrast, minimal coreplication of a Col E1- and a pMB1-derivative plasmid occurred, with the latter type rapidly excluding the former. We suggest here that the pMB1 derivatives, pMB9 and pBR322, are less sensitive than Col E1 derivatives to the putative inhibitor that regulates plasmid replication, due to base sequence differences in their target for the inhibitor, and consider one mechanism whereby the duplication of Col E1-like plasmids might be regulated.

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Citations

Jan 1, 1989·Molecular & General Genetics : MGG·H YasuedaT Itoh
Feb 1, 1984·Gene·G BradyT Hashimoto-Gotoh
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J Tomizawa, T Itoh
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H DanbaraK N Timmis
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Som, J Tomizawa
Jan 24, 2006·Journal of Bacteriology·Masaru YaguraTateo Itoh
Oct 30, 2004·The Journal of Reproduction and Development·Masahiko FujisawaYoshihiro Hayashi
Jun 26, 2012·Protein Expression and Purification·Venuka DuraniThomas J Magliery
Feb 25, 1983·Journal of Molecular Biology·J W ChaseJ J Sninsky
Jan 1, 1987·Plasmid·C M LambertP Strike
Jun 25, 2015·Microbiology Spectrum·Chang-Ho BaekFederico Katzen

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